EurekaMag.com logo
+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Excision of an 11-kilobase-pair DNA element from within the nifD gene in anabaena variabilis heterocysts



Excision of an 11-kilobase-pair DNA element from within the nifD gene in anabaena variabilis heterocysts



Journal of Bacteriology 171(8): 4138-4145



The 3' region of the Anabaena variabilis nifD gene contains an 11-kilobase-pair element which is excised from the chromosome during the heterocyst differentiation. We have sequenced the recombination sites which border the element in vegetative cells and the rearranged heterocyst sequences. In vegetative cells, the element was flanked by 11-base-pair direct repeats which were identifical to the repeats present at the ends of the nifD element in Anabaena sp. strain PCC 7120 (Anabaena strain 7120). Although Anabaena strain 7120 and A. variabilis are quite distinct in many ways, the overall sequence similarity between the two strains for the regions sequenced was 96%. Like the Anabaena strain 7120 element, the A. variabilis element was excised in heterocysts to produce a functional nifD gene and a free circularized element which was neither amplified nor degraded. The Anabaena strain 7120 xisA gene is located at the nifK-proximal end of the nifD element and is required for excision of the element in heterocysts. The A. variabilis element also contained an xisA gene which could complement a defective Anabaena strain 7120 xisA gene. A. variabilis did not contain the equivalent of the Anabaena strain 7120 fdxN 55-kilobase-pair element.

(PDF emailed within 1 workday: $29.90)

Accession: 001831105

Download citation: RISBibTeXText

PMID: 2502534



Related references

Expression of the Anabaena sp. strain PCC 7120 xisA gene from a heterologous promoter results in excision of the nifD element. Journal of Bacteriology 172(7): 3925-3931, 1990

Estimation of gene expression in heterocysts of Anabaena variabilis by using DNA-RNA hybridization. Journal of Bacteriology 167(3): 940-946, 1986

Excision of Anabaena PCC 7120 nifD element in Escherichia coli: Growth kinetics and RecA regulated xisA expression and DNA rearrangement. Bioresource Technology 99(11): 4551-4558, 2007

Alkane-induced expression, substrate binding profile, and immunolocalization of a cytochrome P450 encoded on the nifD excision element of Anabaena 7120. Bmc Microbiology 5: 16-16, 2005

Deletion of a 55-kilobase-pair DNA element from the chromosome during heterocyst differentiation of Anabaena sp. strain PCC 7120. Journal of Bacteriology 170(11): 5034-5041, 1988

Evolution and utilization of hydrogen by the cultures of isolated heterocysts of cyanobacteria Anabaena variabilis, Anabaena cylindrica, Algae. Applied biochemistry and microbiology 18(3): 248-254, 1982

Genetic evidence of that hepA gene is involved in the normal deposition of the envelope of both heterocysts and akinetes in Anabaena variabilis ATCC 29413. FEMS Microbiology Letters 123(1-2): 63-67, 1994

Distinct and differently regulated Mo-dependent nitrogen-fixing systems evolved for heterocysts and vegetative cells of Anabaena variabilis ATCC 29413: characterization of the fdxH1/2 gene regions as part of the nif1/2 gene clusters. Molecular Microbiology 18(2): 357-369, 1995

Transcriptional activity of heterocysts isolated from anabaena variabilis. Archives of Microbiology 148(2): 115-120, 1987

Presence of phycobilins in heterocysts isolated from anabaena variabilis. Plant Physiology (Rockville) 63(5 SUPPL): 28, 1979

Isolated heterocysts of Anabaena variabilis synthesize envelope polysaccharide. Biochimica et Biophysica Acta 674(2): 265-276, 1981

Dna content of heterocysts and spores of the filamentous cyanobacterium anabaena variabilis. FEMS Microbiology Letters 8(4): 241-245, 1980