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Existence of mixed isotype A beta E alpha class II molecules in Ed alpha gene-introduced C57BL/6 transgenic mice



Existence of mixed isotype A beta E alpha class II molecules in Ed alpha gene-introduced C57BL/6 transgenic mice



Immunology 69(3): 385-390



In this study the existence of mixed isotype A.beta.b/E.alpha.d molecules in Exd gene-introduced C57BL/6 (B6E.alpha.d) transgenic mice is demonstrated. Biosynthetically labelled B6E.alpha.d transgenic spleen cells were immunoprecipitated with anti-A.beta.b or anti-E.alpha.d monoclonal antibody (mAb) and analysed by two-dimensional gel electrophoresis (NEPHGE/SDS-PAGE). Anti-A.beta.b mAb precipitated E.alpha.d molecules in addition to A.beta.b and A.alpha.b molecules. Anti-E.alpha.d mAb precipitated A.beta.b molecules in addition to E.beta.b and E.alpha.d molecules. No E.alpha.d molecule was precipitated by anti-A.beta.b mAb from B10.A(3R) spleen cells, which have a very similar organization of class II molecules to B6E.alpha.d transgenic mice. Since this B6E.alpha.d transgenic mouse was shown to have 20-40 copies per cell of the E.alpha.d transgene (Kashiwamura et al., 1988), it is speculated that large amounts of E.alpha.d transgene are made but, according to the data presented here, only a small amount actually associated with A.beta.b as opposed to E.beta.b.

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Accession: 001831211

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PMID: 2312163



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