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Experiment on the in vitro culture of spring swede rape

Experiment on the in vitro culture of spring swede rape

Sibirskii Vestnik Sel' skokhozyaistvennoi Nauki ( 3): 42-45

When explants from various organs of 3 varieties were cultured, the best results were obtained by preliminary sterilization of the seeds in a 10% solution of chloramine B for 30 min before germination. Stem explants about 5 mm in size with apical and axillary buds were cultured on MS and Gamborg B5 media without growth regulators and with half the normal concentration of salts. Culture on B5 agar medium gave clones suitable for planting in soil in 3-4 weeks.

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