+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Heat-shock disruption of trans-splicing in trypanosomes: effect on Hsp70, Hsp85 and tubulin mRNA synthesis

Heat-shock disruption of trans-splicing in trypanosomes: effect on Hsp70, Hsp85 and tubulin mRNA synthesis

Gene 82(1): 169-175

The synthesis of most, if not all, mRNA in trypanosomes involves the splicing in trans of two RNA molecules. One of these includes the common 5' mini-exon or spliced-leader sequence, while the other contains the complete protein-coding exon sequence for a given gene. We have recently shown that trans-splicing of tubulin transcripts is disrupted by heat shock, whereas trans-splicing of Hsp70 mRNA continues unabated, despite the increased expression of this latter gene [Muhich and Boothroyd, Mol. Cell Biol. 8 (1988) 3837-3846]. In this report we show that, in addition to being polyadenylated, the dimeric beta-alpha tubulin transcripts which accumulate as a result of heat shock have a correctly processed 5'-end (through mini-exon addition), and that the extent of disruption is proportional to the degree of heat shock. We further show that mRNA levels for two evolutionarily-conserved heat-shock proteins, Hsp70 and Hsp85, are increased under the splicing-disruptive conditions of heat shock.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 001850197

Download citation: RISBibTeXText

PMID: 2684772

DOI: 10.1016/0378-1119(89)90042-5

Related references

Heat shock disruption of trans splicing in trypanosomes effect on hsp70 hsp85 and tubulin messenger rna synthesis. Gene 82(1): 169-176, 1989

Synthesis of trypanosome hsp70 mRNA is resistant to disruption of trans-splicing by heat shock. Journal of Biological Chemistry 264(13): 7107-7110, 1989

Synthesis of trypanosome hsp70 messenger rna is resistant to disruption of trans splicing by heat shock. Journal of Cellular Biochemistry Suppl. (13 Part E): 85, 1989

A common pyrimidine-rich motif governs trans-splicing and polyadenylation of tubulin polycistronic pre-mRNA in trypanosomes. Genes and Development 8(4): 491-501, 1994

Heat-shock proteins Hsp104 and Hsp70 reactivate mRNA splicing after heat inactivation. Current Biology 5(3): 306-317, 1995

Post-transcriptional repair of a split heat shock protein 90 gene by mRNA trans-splicing. Journal of Biological Chemistry 286(9): 7116-7122, 2011

Polycistronic transcripts in trypanosomes and their accumulation during heat shock: evidence for a precursor role in mRNA synthesis. Molecular and Cellular Biology 8(9): 3837-3846, 1988

Trans-splicing to spliceosomal U2 snRNA suggests disruption of branch site-U2 pairing during pre-mRNA splicing. Molecular Cell 26(6): 883-890, 2007

The heat-shock phenomenon in cultured cells of rainbow trout hsp70 mRNA synthesis and turnover. Biochimica et Biophysica Acta - Gene Structure and Expression 783(2): 137-143, 1984

Induction of heat shock hsp70 mRNA and HSP70 kDa protein in neurons in the penumbra following focal cerebral ischemia in the rat. Brain Research. 619(1-2): 334-338, 1993

MRNA maturation by two-step trans-splicing/polyadenylation processing in trypanosomes. Proceedings of the National Academy of Sciences of the United States of America 104(7): 2035-2042, 2007

Temporal order of RNA-processing reactions in trypanosomes: rapid trans splicing precedes polyadenylation of newly synthesized tubulin transcripts. Molecular and Cellular Biology 13(1): 720-725, 1993

Cloning of the heat shock protein 70 (HSP70) gene of Ehrlichia sennetsu and differential expression of HSP70 and HSP60 mRNA after temperature upshift. Infection and Immunity 66(7): 3106-3112, 1998

Binding of heat-shock protein 70 (hsp70) to tubulin. Archives of Biochemistry and Biophysics 310(2): 428-432, 1994

Expression of HSP70 mRNA and MDR1 mRNA in K562 cells induced by heat shock and ADM. Zhongguo Shi Yan Xue Ye Xue Za Zhi 15(6): 1169-1172, 2007