+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Identification of two groups of leghemoglobin genes in alfalfa (Medicago sativa) and a study of their expression during root nodule development



Identification of two groups of leghemoglobin genes in alfalfa (Medicago sativa) and a study of their expression during root nodule development



Plant Molecular Biology 11(6): 761-772



Differential screening of an alfalfa root nodule cDNA library with either root or nodule mRNA resulted in the isolation of two groups of leghemoglobin cDNA which differ significantly in sequence. Analysis of one member of each group revealed a divergence within the coding region of 15% at the nucleotide level and 14% at the amino acid level. The 3' non-coding sequences are 25% divergent but are highly conserved over a stretch of 54 nucleotides which contains two sequence motifs common to leghemoglobin genes from other plant species. Southern blotting analysis with exon-specific probes has shown that there are approximately twice as many leghemoglobin gene copies in the alfalfa genome corresponding to one type of cDNA as compared with the other. Using the same criterium of DNA sequence relatedness these two distinct groups of leghemoglobin genes have also been identified in the genomes of the diploid annual Medicago truncatula and the closely related genus, Melilotus. Transcripts corresponding to both groups of leghemoglobin genes are first detected in alfalfa nodules 9-10 days after Rhizobium inoculation. Thereafter, mRNA levels increase rapidly and synchronously, reaching a maximum approximately 2 days later. There is a 2-3 fold difference in the steady-state levels of the two mRNA populations and this is maintained throughout the subsequent two weeks of nodule growth. The absence of any detectable transcription during the early stages of nodule development and the apparent co-ordinate expression of leghemoglobin genes in alfalfa contrasts with the situation in soybean and suggests that important differences in leghemoglobin gene regulation exist between these two distantly related legume species.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001855809

Download citation: RISBibTeXText

PMID: 24272626

DOI: 10.1007/bf00019516


Related references

Expression of leghemoglobin genes of alfalfa medicago sativa l. vernal during root nodule development. Bothe, H , F J De Bruijn And W E Newton (Ed ) Nitrogen Fixation: Hundred Years After; 7th International Congress, Cologne, West Germany, March 13-20, Xxi+878p Vch Publishers, Inc : New York, New York, Usa; Vch Verlagsgesellschaft Mbh: Weinheim, West Germany; Gustav Fischer Verlag: Stuttgart, West Germany Illus 636, 1988

Molecular cloning of alfalfa medicago sativa leghemoglobin structural genes synthesized by polyadenylated messenger rna from alfalfa root nodules. Korean Biochemical Journal 18(1): 77-81, 1985

Expression of a homeotic gene during alfalfa Medicago sativa root nodule development. Palacios, R, Mora, J, Newton, W E Current Plant Science and Biotechnology in Agriculture; New horizons in nitrogen fixation 344, 1993

Transcription and translation of alfalfa leghemoglobin genes during root nodule development. Science in China Series B Chemistry Life Sciences & Earth Sciences 33(9): 1078-1083, 1990

The structure and evolution of genes for leghemoglobin and nodulin 25 of alfalfa medicago sativa l. Bothe, H. De Bruijn And W. E. Newton (ed.). Nitrogen Fixation: Hundred Years After; 7th International Congress, Cologne, West Germany, March 13-20, . Xxi 878p. Vch Publishers, Inc: New York, New York, Usa; Vch Verlagsgesellschaft Mbh: Weinheim, West Germany; Gustav Fischer Verlag: Stuttgart, West Germany. Illus. 646, 1988

Leghemoglobin content of alfalfa medicago sativa root nodules under water stress. Plant Physiology 77(SUPPL 4): 162, 1985

Purification of leghemoglobin messenger rna from alfalfa medicago sativa root nodules and in vitro translation. Korean Biochemical Journal 18(1): 71-76, 1985

Variation in the relationship between nitrogen fixation, leghemoglobin, nodule numbers and plant biomass in alfalfa (Medicago sativa) caused by treatment with arsenate, heavy metals and fluoride. Physiologia Plantarum 57(4): 579-583, 1983

Leghemoglobins from alfalfa (Medicago sativa L. Vernal) root nodules. I. Purification and in vitro synthesis of five leghemoglobin components. Plant science letters 25(2): 118-132, 1982

Identification of genes induced during Medicago sativa nodule development by using the cDNA-AFLP technique. CHINESE SCIENCE BULLETIN 51(17): 2087-2094, 2006

Alfalfa medicago sativa root nodule carbon di oxide fixation. Plant Physiology 72(2): 469-473, 1983

Paenibacillus medicaginis sp. nov. a chitinolytic endophyte isolated from the root nodule of alfalfa (Medicago sativa L.). International Journal of Systematic and Evolutionary Microbiology (): -, 2015

In vitro translation of nodule specific messenger rna from alfalfa medicago sativa root nodules. Korean Biochemical Journal 19(2): 203-206, 1986

Paenibacillus medicaginis sp. nov. a chitinolytic endophyte isolated from a root nodule of alfalfa (Medicago sativa L.). International Journal of Systematic and Evolutionary Microbiology 65(11): 3853-3860, 2015

Symbiosis between the root-nodule bacteriumSinorhizobium melilotiand alfalfa (Medicago sativa) under salinization conditions. Microbiology 75(2): 242-242, 2006