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In vitro fertilization of bovine follicular oocytes recovered by laparoscopy



In vitro fertilization of bovine follicular oocytes recovered by laparoscopy



Theriogenology 31(4): 927-934



Thirty-three laparoscopies were performed on 16 follicle stimulating hormone (FSH)- superovulated cows (one to four laparoscopies per cow). Two hundred and four follicles were aspirated (6.18/cow) and 157 oocytes (77% recovery rate) were isolated. Sixty percent of the oocytes found were considered to be of good to excellent quality. In cows with ongoing ovulations at laparoscopy only 32% of the oocytes were suitable for fertilization. Twenty-four percent of the oocytes from cows with no ovulation and 16% from cows with ovulations were fertilized. Higher proportions of oocytes were fertilized in cows showing estrous behavior vs cows with no signs of estrus (25 vs 15%). Using lysolecithin- treated spermatozoa, 28% of the oocytes with good to excellent quality were fertilized, and 35% of the fertilized eggs cleaved in vitro to two-to-four-cell stages.

Accession: 001859074

Download citation: RISBibTeXText

PMID: 16726608

DOI: 10.1016/0093-691x(89)90038-1

Download PDF Full Text: In vitro fertilization of bovine follicular oocytes recovered by laparoscopy



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