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Investigation of benzo(a)pyrene and aflatoxin B1-DNA adducts by indirect immunofluorescence

Investigation of benzo(a)pyrene and aflatoxin B1-DNA adducts by indirect immunofluorescence

Proceedings Annual Meeting of the American Association for Cancer Research 31: 90

Antibodies generated against benzo(a)pyrene diol epoxide modified DNA were used to investigate adducts in skin biopsies of coal tar treated psoriasis patients. Specific nuclear staining was visible in the epidermal cells of all biopsies from patients but not from untreated controls. Postlabelling of DNA isolated from the biopsies was used to determine the spectrum of hydrophobic adducts present. A pattern of multiple adducts was detected in the samples obtained from treated patients.

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Detection of benzo a pyrene dna adducts in cultured cells treated with benzo a pyrene diol epoxide by quantitative immunofluorescence microscopy and phosphorus 32 postlabeling immunofluorescence analysis of benzo a pyrene dna adducts in bronchial cells from smoking individuals. Bartsch, H , K Hemminki And I K O'neill (Ed ) IARC (International Agency For Research on Cancer) Scientific Publications, No 89 Methods For Detecting Dna Damaging Agents in Humans: Applications in Cancer Epidemiology And Prevention; Symposium on Detection Of Dna-Damaging Agents in Man, Espoo, Finland, September 2-4, 1987 Xi+518p Oxford University Press: New York, New York, Usa; Oxford, England, Uk; International Agency For Research on Cancer: Lyon, France Illus Paper 146-154, 19, 1988

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