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Is the Suppressor-mutator element controlled by a basic developmental regulatory mechanism?


, : Is the Suppressor-mutator element controlled by a basic developmental regulatory mechanism?. Genetics 120(2): 559-577

We report the results of genetic studies on derivatives of two different alleles of the maize a locus with an insertion of the Suppressor-mutator (Spm) transposable element in which the element is inactive, but can be reactivated readily. We present evidence that the mechanism that determines whether the element is in an active or inactive phase has two genetically distinguishable components. One determines whether or not the element is genetically active (the phase setting) and the other determines the stability of the setting in development, its heritability, and its phase in the next generation (the phase program). We show that the element's phase can be reset in a reproducible pattern during plant development. We also show that the Spm element can be reprogrammed to undergo a subsequent phase change without a concomitant phase change. The capacity to reset and reprogram the Spm element is differentially expressed within the plant in a pattern that is correlated with the developmental fate of apical and lateral meristems, suggesting the involvement of a basic developmental determination mechanism.

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Accession: 001871135

PMID: 2848747

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Related references

Fedoroff, N.; Banks, J.A.; Masson, P., 1989: Molecular genetic analysis of the maize Suppressor-mutator element's epigenetic developmental regulatory mechanism. Previous work and published studies on the genetic analysis of the mechanism underlying the developmental regulation of the maize Suppressor-mutator (Spm) element are reviewed. It is concluded that the element is subject to inactivation by a negat...

Banks, J.A.; Masson, P.; Fedoroff, N., 1988: Molecular mechanisms in the developmental regulation of the maize Suppressor-mutator transposable element. The maize Suppressor-mutator (Spm) element can exist in one of three heritable forms: (1) a stably active form, (2) a stably inactive form, termed cryptic, and (3) a labile form, here termed programmable, in which the element exhibits one of a var...

Banks J.A.; Fedoroff N., 1989: Patterns of developmental and heritable change in methylation of the suppressor mutator transposable element. Genetic inactivation of the Suppressor-mutator [Spm] element is correlated with methylation of sequences surrounding the element's transcription initiation site. Several stages in the development of the plant have been identified during which...

Fedoroff, N.; Masson, P.; Banks, J.A., 1989: Mutations, epimutations, and the developmental programming of the maize Suppressor-mutator transposable element. The review explains what the characterization of deletion mutations, epimulations (reversible genetic changes) and the methylation status of the element reveal about its mode of transposition. The element is sunbject to an epigenetic mechanism tha...

Schiefelbein, J.W.; Raboy, V.; Fedoroff, N.V.; Nelson, O.E.J., 1985: Deletions within a defective suppressor-mutator element in maize affect the frequency and developmental timing of its excision from the bronze locus. Six independent derivatives of the bz-m13 allele, which contains a 2.2 kbp defective suppressor-mutator (dSpm) insertion at the bronze (bz) locus, were isolated and analysed. All show somatic reversion (to normal pigmentation due to the excision o...

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Fedoroff N.; Masson P.; Banks J., 1988: Regulation of the maize suppressor mutator element. Lambert, M E , J F Mcdonald And I B Weinstein (Ed ) Banbury Report, Vol 30 Eukaryotic Transposable Elements As Mutagenic Agents; Conference, Cold Spring Harbor, New York, Usa, April 21, 1987 Xvi+345p Cold Spring Harbor Laboratory: Cold Spring Harbor, New York, Usa Illus 63-70

Fedoroff, N.V., 1999: The suppressor-mutator element and the evolutionary riddle of transposons. This review focuses on the epigenetic control of the maize Suppressor-mutator (Spm) transposon and the evolutionary origin of epigenetic mechanisms. Methylation of the Spm promoter prevents transcription and transposition, and the methylation of t...

Fedoroff, N.M.sson, P.B.nks, J.K.ngsbury, J., 1988: Positive and negative regulation of the suppressor-mutator element. Plant transposable elements edited by Oliver Nelson