EurekaMag.com logo
+ Translate

Isolation and characterization of a cysteine proteinase from Fasciola hepatica adult worms


, : Isolation and characterization of a cysteine proteinase from Fasciola hepatica adult worms. Molecular and Biochemical Parasitology, 351: 89-95

Adult Fasciola hepatica worms contain multiple proteinases capable of degrading hemoglobin, immunoglobulins and collagen. Here we report the isolation and biochemical characterization of a cysteine proteinase from acidic extracts of these worms. The enzyme was purified to homogeneity by cation exchange and molecular sieve high-performance liquid chromatography. It eluted at a native molecular weight of approximately 14,500 and migrated as a single band at approximately 14,500 Da upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Activity was assessed by employing synthetic peptide substrates, such as carbobenzoxy-phenylalanyl-arginyl-7-amino-4-trifluoro-methylcoumarin, commonly used to assay other cysteine proteinases. The proteinase was maximally active at pH 6.0, with 50% or more of the activity detected between pH 4.5 and 7.5. Inhibition of activity at pH 5.5 was seen only with compounds known to inhibit cysteine proteinases. No effect was seen with inhibitors of aspartic, serine, or metalloproteinases. The purified enzyme was stable at acidic pH at 4 degrees C, 25 degrees C, -20 degrees C, and in 1 M urea.

Accession: 001871368

PMID: 2761575

DOI: 10.1016/0166-6851(89)90146-1

Download PDF Full Text: Isolation and characterization of a cysteine proteinase from Fasciola hepatica adult worms


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Rege, A.A.; Herrera, P.R.; Lopez, M.; Dresden, M.H., 1989: Isolation and characterization of a cysteine proteinase from Fasciola hepatica adult worms. Adult Fasciola hepatica worms contain multiple proteinases capable of degrading hemoglobin, immunoglobulins and collagen. Here we report the isolation and biochemical characterization of a cysteine proteinase from acidic extracts of these worms. T...

Ruiz, A.; Molina, J.M.; Gonzalez, J.F.; Camara, S.; Redondo, E.S.H., 2000: IgG response of goats experimentally infected with Fasciola hepatica against a cysteine protease of adult worms. Acta Parasitologica 45(3): 201, July

Fagbemi, B.O.; Hillyer, G.V., 1992: The purification and characterization of a cysteine protease of Fasciola gigantica adult worms. A 26-28 kDa protease was isolated from Fasciola gigantica adult worms by a two-stage purification process of column chromatography in a Sephacryl S-200 column and affinity chromatography in an L-phenylalanine-agarose column. This protease is a cys...

Fagbemi, BO.; Hillyer, GV., 1992: The purification and characterization of a cysteine protease of Fasciola gigantica adult worms. A 26-28 kDa protease was isolated from Fasciola gigantica adult worms by a two-stage purification process of column chromatography in a Sephacryl S-200 column and affinity chromatography in an L-phenylalanine-agarose column. This protease is a cys...

Song, C.Y.; Kim, T.S., 1994: Characterization of a cysteine proteinase from adult worms of Paragonimus westermani. Paragonimus westermani, the lung fluke, is known to migrate to the pulmonary tissue of mammalian hosts and causes pathological changes in the lungs. An acidic thiol-dependent proteinase with a molecular weight of approximately 20,000 daltons was p...

Song, C.Y.; Dresden, M.H.; Rege, A.A., 1990: Clonorchis sinensis: purification and characterization of a cysteine proteinase from adult worms. 1. Adult Clonorchis sinensis, the Chinese liver fluke, is known to migrate to the bile ducts of its mammalian host and cause significant pathology. 2. An acidic, thiol-dependent proteinase with a native mol. wt of approximately 18,500 was purified...

Yang, S.Hoon.; Park, J-Ok.; Lee, J.Hwa.; Jeon, B.Hun.; Kim, W.Sin.; Kim, S-Il.; Yun, K-Jung.; Jeong, E.Taik.; Lee, K-Whoan.; Kim, Y.Man.; Lee, M.Hyang.; Park, H., 2004: Cloning and characterization of a new cysteine proteinase secreted by Paragonimus westermani adult worms. The cysteine proteinases of Paragonimus westermani are known to play important roles in invasion and pathogenesis to hosts and in immune modulation and nutrient uptake. In this study, we have cloned a new cysteine proteinase of P. westermani, PwCP...

Dowd, A.J.; Smith, A.M.; Carmona, C.; Robertson, C.; Dalton, J.P., 1992: Purification and characterisation of a cysteine proteinase secreted by Fasciola hepatica. Biochemical Society Transactions 20(1): 86s-86s

Kofta, W.; Mieszczanek, J.; Wedrychowicz, H., 2000: Differential reaction to DNA vaccination with cDNA of Fasciola hepatica cysteine proteinase. Acta Parasitologica 45(3): 204, July

Wedrychowicz, H.; Kofta, W.; Mieszczanek, J.; Klockiewicz, M.W., 2000: Investigation of cysteine proteinase as a potential vaccine candidate against fasciola hepatica infection. Immunology Letters 73(2-3): 272, September