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Isolation and characterization of a new antigen (CS1541) from a porcine enterotoxigenic Escherichia coli O8:KX105 strain


, : Isolation and characterization of a new antigen (CS1541) from a porcine enterotoxigenic Escherichia coli O8:KX105 strain. FEMS Microbiology Letters 55(3): 341-347

A fimbrial antigen (CS1541) was extracted and purified from the porcine enterotoxigenic strain 1541(O8:K-:H9). CS1541 fimbriae appeared as long thin filaments 3-5 mm in diameter. CS1541 antigen consisted of two peptide bands of about 18 and 19 kDa as demonstrated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting. It was expressed both at 37 degrees C and 15 degrees C and did not have haemagglutinating properties.


Accession: 001871385

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Related references

Broes, A.F.irbrother, J.; Jacques, M.L.riviere, S., 1988: Isolation and characterization of a new fimbrial antigen (CS1541) from a porcine enterotoxigenic Escherichia coli O8: KX105 strain. FEMS microbiology letters Federation of European Microbiological Societies, 55(3): 341-348

Broes, A.; Fairbrother, J.M.; Jacques, M.; Larivière, S., 1989: Requirement for capsular antigen KX105 and fimbrial antigen CS1541 in the pathogenicity of porcine enterotoxigenic Escherichia coli O8:KX105 strains. The requirement for capsular antigen KX105 and fimbrial antigen CS1541 in the pathogenicity of porcine enterotoxigenic Escherichia coli O8:KX105 strains lacking the colonization factor antigens K88, K99, 987P and F41 was investigated using two enc...

Broes, A.; Fairbrother, J.M.; Jacques, M.; Lariviere, S., 1989: Requirements for capsular antigen KX105 and fimbrial antigen CS1541 in the pathogenicity of porcine enterotoxigenic Escherichia coli O8: KX105 strains. The pathogenicity of porcine enterotoxogenic E. coli O8:KX105 strains lacking the colonization factor antigens K88, K99, 987P and F41 was investigated using two encapsulated strains and their acapsular variants, one of which produced the fimbrial...

Broes, A.; Fairbrother, J.M.; Mainil, J.; Harel, J.; Lariviere, S., 1988: Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli serotype O8:KX105 and O8:K"2829" strains isolated from piglets with diarrhea. Twelve pathogenic and seven nonpathogenic enterotoxigenic Escherichia coli strains which were previously identified as belonging to serogroup O8:KX105 (A. Broes, J. M. Fairbrother, S. Larivière, M. Jacques, and W. M. Johnson, Infect. Immun. 56:24...

Broes, A.; Fairbrother, J.M.; Mainil, J.; Harel, J.; Lariviere, S., 1988: Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli serotype O8:KX105 and O8:K2829 strains isolated from piglets with diarrhea. Twelve pathogenic and 7 non-pathogenic enterotoxigenic Escherichia coli strains previously identified as serogroup O8:KX105 were examined. Only the 12 pathogenic strains were confirmed to possess the capsular antigen KX105. The 7 non-pathogenic st...

Broes A.; Fairbrother J.M.; Mainil J.; Harel J.; Lariviere S., 1988: Phenotypic and genotypic characterization of enterotoxigenic escherichia coli serotype o8 kx105 and o8 k 2829 strains isolate from piglets with diarrhea. Twelve pathogenic and seven nonpathogenic enterotoxigenic Escherichia coli strains which were previously identified as belonging to serogroup O8:KX105 (A. Broes, J. M. Fairbrother, S. Lariviere, M. Jacques, and W. M. Johnson, Infect. Immun. 56:241...

González, E.A.; Vázquez, F.; Ignacio Garabal, J.; Blanco, J., 1995: Isolation of K88 antigen variants (ab, ac, ad) from porcine enterotoxigenic Escherichia coli belonging to different serotypes. Fimbriae isolation by means of thermal shock was applied to 15 K88-positive (3 K88ab, 9 K88ac and 3 K88ad) E. coli reference strains belonging to serotypes O8:K87, O32, O45, O138:K81, O141:K85, O147:K89, O149:K91 and O157, as well as to 10 K88-pos...

Altboum, Z.; Levine, M.M.; Galen, J.E.; Barry, E.M., 2003: Genetic characterization and immunogenicity of coli surface antigen 4 from enterotoxigenic Escherichia coli when it is expressed in a Shigella live-vector strain. The genes that encode the enterotoxigenic Escherichia coli (ETEC) CS4 fimbriae, csaA, -B, -C, -E, and -D', were isolated from strain E11881A. The csa operon encodes a 17-kDa major fimbrial subunit (CsaB), a 40-kDa tip-associated protein (CsaE...

Dima, V.F.; Petrovici, A.; Dima, S.V., 1990: Characterization of colonization factor antigen CFA/I from an enterotoxigenic Escherichia coli strain (0128:H12). CFA/I antigen was isolated and purified from E. coli, mutant 279 B-1-14, serotype 0128:H12, and had the following biochemical and biological features: a) amino-acid content was similar to that of purified antigen prepared from strain H10407; b) la...

Vázquez, Fán.; González, E.A.; Garabal, Jé.I.; Blanco, J., 2007: Characterization of fimbriae extracts from porcine enterotoxigenic Escherichia coli strains carrying F6 (987P) antigen. Fimbrial extracts from porcine enterotoxigenic Escherichia coli (ETEC) strains carrying F6 (987P) intestinal colonization factor antigen wereobtained using the thermal shock method. The extracts were analyzed by SDSPAGE and immunoblotting using di...