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Isolation and characterization of cleaved prolactin generated by target tissue


, : Isolation and characterization of cleaved prolactin generated by target tissue. Dissertation Abstracts International B, Sciences and Engineering 47(8): 3262

The ability of cleaved prolactin (PRL) to displace 125I-oPRL from rat ventral prostate or female rat liver membranes was indistinguishable from that of intact PRL. Similarly, intact PRL and cleaved PRL were equipotent in their ability to stimulate the proliferation of Nb2 lymphoma cells. The supernatant was purified by a hydrophobic interactive column with HPLC techniques, and eluates reanalysed and quantitated by Coomassie Blue stained SDS gels.

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Vick, R.S.; Wong, V.L.; Witorsch, R.J., 1987: Biological, immunological and biochemical characterization of cleaved prolactin generated by lactating mammary gland. We have previously shown that rat prolactin is proteolytically cleaved in its loop by peripheral tissues of the rat. Of the tissues examined to date, lactating mammary gland exhibits the highest prolactin-cleaving activity. The objective of this s...

Casabiell, X.; Robertson, M.C.; Friesen, H.G.; Casanueva, F.F., 1989: Cleaved prolactin and its 16K fragment are generated by an acid protease. A cleaved form of rat PRL (cldPRL), which resolves after reduction of the disulfide bonds into two fragments of 16K and 8K mol wt, has been reported in the pituitary gland. To further understand the genesis of this cleaved PRL and its related 16K...

Andries, M.; Jacobs, G.F.; Tilemans, D.; Denef, C., 1996: In vitro immunoneutralization of a cleaved prolactin variant: evidence for a local paracrine action of cleaved prolactin in the development of gonadotrophs and thyrotrophs in rat pituitary. We have previously isolated a cleaved prolactin variant, secreted by rat pituitary cells in culture, that stimulated [3H]thymidine incorporation into DNA in gonadotrophs and thyrotrophs when added to pituitary aggregate cell cultures of 14-day-old...

Andries, M.; Jacobs, G., F.M.; Tilemans, D.; Denef, C., 1996: In vitro immunoneutralization of a cleaved prolactin variant: Evidence for a local paracrine action of cleaved prolactin in the development of gonadotrophs and thyrotrophs in rat pituitary. We have previously isolated a cleaved prolactin variant, secreted by rat pituitary cells in culture, that stimulated [3H]thymidine incorporation into DNA in gonadotrophs and thyrotrophs when added to pituitary aggregate cell cultures of 14-day-old...

Hu, Z.Z.; Meng, J.; Dufau, M.L., 2001: Isolation and characterization of two novel forms of the human prolactin receptor generated by alternative splicing of a newly identified exon 11. We have identified a novel exon 11 of the human prolactin receptor (hPRLR) gene that is distinct from its rodent counterparts and have demonstrated the presence of two novel short forms of the hPRLR (S1< sub>a</ sub> and S1< sub>...

Clapp, C.; Sears, P.S.; Russell, D.H.; Richards, J.; Levay-Young, B.K.; Nicoll, C.S., 1988: Biological and immunological characterization of cleaved and 16K forms of rat prolactin. A cleaved form of rat PRL (rPRL) has been reported to exist in the pituitary gland and to be the main product of PRL proteolysis by its target tissues. A 16K fragment derived from cleaved PRL (cPRL) has mammary mitogenic activity in rats in vivo,...

Mittra, I., 1980: A novel 'cleaved prolactin' in the rat pituitary. I. Biosynthesis, characterization and regulatory control. Prolactin cleaved within the large disulphide loop was synthesized and secreted by cultured rat pituitary glands labelled with [35S]methionine, and was isolated by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis and characterized...

Mittra, I., 1980: A novel "cleaved prolactin" in the rat pituitary: part I. Biosynthesis, characterization and regulatory control. Biochemical and Biophysical Research Communications 95(4): 1750-1759

Baldocchi, R.A.; Chou, Y.C.; Nicoll, C.S., 1994: Cleavage of rat prolactin occurs within rat mammary tissue, and the cleaved form is present in serum but not in milk. The present study was undertaken to determine whether cleavage of rat prolactin (rPRL) occurs within rat mammary tissue and to investigate whether the cleaved form and its 16-kDa amino terminal fragment are present in rat serum and milk. The relat...

Clapp, C., 1987: Analysis of the proteolytic cleavage of prolactin by the mammary gland and liver of the rat: characterization of the cleaved and 16K forms. A cleaved form of PRL (CPRL) has been reported to exist naturally in the pituitary and to be the main product of PRL proteolysis by its target tissues. A 16K fragment derived from CPRL has mammary mitogenic activity. To analyze the possible functi...