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Isolation and properties of protoplast from endosperm of developing wheat grain

, : Isolation and properties of protoplast from endosperm of developing wheat grain. Plant Science Limerick 65(1): 55-62

A systematic evaluation of methods for the isolation of viable protoplasts from the endosperm tissue of developing grains of Mardler showed that the optimum involved an enzyme mixture of 1% pectinase and 2% cellulase in 0.3 M mannitol and 0.2 M sucrose as osmoticum at pH 5.5, an incubation time of 2 h at 28 degrees C or 16 h at 4 degrees C, and 1 x g sedimentation for protoplast purification. Centrifugation and abrupt changes in pH or temperature were avoided to prevent protoplast lysis.

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