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Isolation and purification of malaria parasites and their antigens


, : Isolation and purification of malaria parasites and their antigens. Malaria: principles and practice of malariology Volume 1: 349-377

The isolation and purification of asexual stages of malaria parasites, the isolation and purification of sexual stages and sporozoites, and the isolation of constituents from the cell surface of isolated parasites, from parasitized erythrocytes and from cultures are discussed.

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Related references

Vanderberg, J.P., 1980: Isolation and purification of sporozoites of the malaria parasites. Procedures for the isolation and purification of Plasmodium sporozoites are described and evaluated. Sporozoites are often prepared by homogenizing dissected-out salivary glands from infected mosquitoes. They may also be prepared by grinding up wh...

Heidrich, H-G.; Russmann, L.; Bayer, B.; Jung, A., 1979: Free-flow electrophoresis for the separation of malaria infected and uninfected mouse erythrocytes and for the isolation of free parasites (Plasmodium vinckei): a new rapid technique for the liberation of malaria parasites from their host cells. After aggregation of erythrocytes from malaria infected mice, the parasites (Plasmodium vinckei) could be set free using gentle mechanical forces. The mixture of freed parasites, infected and non-infected erythrocytes, and membraneous material was...

Heidrich, H.G.; Rüssmann, L.; Bayer, B.; Jung, A., 1979: Free-flow electrophoresis for the separation of malaria infected and uninfected mouse erythrocytes and for the isolation of free parasites (Plasmodium vinckei): a new rapid technique for the liberation of malaria parasites from their host cells. After aggregation of erythrocytes from malaria infected mice, the parasites (Plasmodium vinckei) could be set free using gentle mechanical forces. The mixture of freed parasites, infected and non-infected erythrocytes, and membraneous material was...

L.W.nlu; Liu Erxiang; Fan Rugong; Mao Yinghong, 1984: Characterization of Plasmodium yoelii antigens by monoclonal antibodies. I. Common antigens between erythrocytic stages of P. yoelii and human malaria parasites. Thirteen cell lines of hybrids secreting monoclonal antibodies (Mabs) against erythrocytic stages of P. yoelii were established by fusing Sp 2/0 myeloma cells with spleen cells of BALB/c mice immunized with a crude preparation of merozoites of the...

Bannister, L.H.; Kent, A.P., 1993: Immunoelectron microscopic localization of antigens in malaria parasites. We present a method for medical image understanding by computer that uses model-based, hierarchical Bayesian inference to accurately segment imaged anatomy. A first application is a prototype system that automatically segments and measures symptom...

Bhattacharya, P.R., 1996: Genetic polymorphism of surface antigens of malaria parasites. The literature on the genetic polymorphism of the surface antigens (circumsporozoite protein and the merozoite surface proteins 1 and 2) of the malaria parasite is reviewed.

Kaslow, D.C.; Duffy, P.E., 1996: Target antigens of transmission blocking antibodies for malaria parasites. Official Gazette of the United States Patent & Trademark Office Patents 1187(3): 1999, June 18

Hollingdale, M.R.; Aikawa, M.; Chen, G.X.; Meis, J.F.; Sakhuja, K.; Sina, B.; Zhu, J.D., 1990: Pre-erythrocytic stage malaria parasites: non-circumsporozoite protein antigens. A series of non-circumsporozoite proteins found in pre-erythrocytic Plasmodium are being developed as putative vaccine candidates. The following antigens are described individually. CSP-2; E12; LSA-1; heat shock proteins; antigens common to exoery...

Kiefer, D.; Halbert, S.P., 1976: Purification of group C streptococcal extracellular antigens detected with naturally occurring human antibodies: isolation of streptokinase and two previously undescribed antigens. Twelve antigens were detected in crude group C streptococcal extracellular concentrates, using naturally occurring antibodies in normal human gamma globulin. These group C streptococcal antigens all appeared to be present in crude group A streptoc...

Anonymous, 1984: Purification of glutathione reductase ec 1642 from different malaria parasites