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Isolation and restriction endonuclease analysis of tetracycline resistance plasmid from Staphylococcus hyicus

, : Isolation and restriction endonuclease analysis of tetracycline resistance plasmid from Staphylococcus hyicus. Veterinary Microbiology 24(2): 113-122

A plasmid of 4550 kb, conferring resistance to tetracycline, was demonstrated in S. hyicus cultures from piglets with exudative epidermitis. The plasmid-encoded properties were determined both by curing and interspecific protoplast transformation experiments. The tetracycline resistance (TET) plasmid, designated pST1, was characterized by restriction endonuclease analysis and a preliminary restriction map was constructed.

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