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Isolation and structure analysis of components from venom of the spider Argiope lobata


, : Isolation and structure analysis of components from venom of the spider Argiope lobata. Toxicon, 275: 541-549

Homologous low molecular weight compounds, blocking postsynaptic glutamate receptors, were isolated from the venom of the spider Argiope lobata by ion exchange chromatography and reverse phase HPLC. Structures of nine blocking agents were determined by 1H-NMR and mass spectroscopy. Two-dimensional COSY spectra were used to identify spin systems of the protons coupled via two or three chemical bonds. The spin systems were combined into the sequence by means of the nuclear Overhauser effect between spatially close protons. Ionogenic groups were identified from pH dependences of the proton chemical shifts. Molecular structures thus obtained were checked against amino acid analysis and mass spectrometry data. The antagonists can be divided into three groups: argiopin, argiopinins and pseudoargiopinins depending on features of the chromophoric moiety (2,4-dihydroxyphenylacetic acid, 4-hydroxy-indole-3-acetic acid or indole-3-acetic acid).

Accession: 001871772

PMID: 2749753

DOI: 10.1016/0041-0101(89)90115-3

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Related references

Grishin, E.V.; Volkova, T.M.; Arseniev, A.S., 1989: Isolation and structure analysis of components from venom of the spider Argiope lobata. Homologous low molecular weight compounds, blocking postsynaptic glutamate receptors, were isolated from the venom of the spider Argiope lobata by ion exchange chromatography and reverse phase HPLC. Structures of nine blocking agents were determin...

Usmanov, P.B.; Kalikulov, D.; Nasirov, K.E.; Akhmedov, K.D.; Nurtaev, K.S., 1991: New neurotoxic components from the venom of the spider Argiope lobata. Two neurotoxic components, differing in their action mechanisms from those of argiopin and argiotoxin, were isolated from the venom of A. lobata and other species of Araneidae. Component 1, A-YB21 and component 2, argiolobatin, blocked, reversibly...

Usmanov P.B.; Kalikulov D.; Nasirov K.E.; Akhmedov K.D.; Nurtaev K.S., 1991: New neurotoxic components of the venom of the spider argiope lobata. Biologicheskie Membrany (Moscow) 8(11): 1135-1136

Usmanov, PB.; Kalikulov, J.; Nasirov, KE.; Akhmedov, KJ.; Nurtaev, HS., 1991: The new neurotoxic components of Argiope lobata spider venom. Biologicheskie Membrany (Moscow): 811: 1135-1136

Grishin, E.V.; Volkova, T.M.; Arsen'ev, A.S., 1988: Glutamate receptor antagonists from the spider Argiope lobata venom. Homologous low molecular weight compounds blocking postsynaptic glutamate receptors were isolated from the Argiope lobata spider venom by ion-exchange chromatography and reverse-phase HPLC. Structures of nine different blocking agents were determi...

Grishin E.V.; Volkova T.M.; Arsen'ev A.S., 1988: Antagonists of glutamate receptors from the venom of argiope lobata spider. Homologous low molecular weight compounds blocking postysynaptic glutamate receptors were isolated from the Argiope lobata spider venom by ion-exchange chromatography and reverse-phase HPLC. Structures of nine different blocking agents were determ...

Usmanov, P.B.; Kalikulov, D.; Shadyeva, N.; Tashmukhamedov, B.A., 1983: Action of the venom of the spider Argiope lobata on glutamatergic and cholinergic synapses. Doklady Akademii Nauk SSSR 273(4): 1017-1018

Usmanov, P.; Kalikulov, D.; Shadyeva, N.; Tashmukhamedov, B., 1983: Effect of the venom of spider Argiope lobata on glutamatergic and cholinergic synapses. Doklady Akademii Nauk SSSR 273(4): 1017-1018

Usmanov, P.; Kalikulov, D.S.adyeva, N.T.shmukhamedov, B., 1984: Action of venom of the spider Argiope lobata on the glutamatergic and cholinergic synapses. Doklady: biological sciences Akademiia nauk SSSR: (pub1984) 732-733

Grishin E.V.; Volkova T.M.; Arsen'ev A.S.; Reshetova O.S.; Onoprienko V.V.; Magazanik L.G.; Antonov S.M.; Fedorova I.M., 1986: Structural and functional characterization of argiopine the ion channel blocker from the venom of the spider argiope lobata. Argiopine, a compound capable of blocking the glutamate-activated ion channels in experiments with glutamatergic synapses of blowfly larvae, was isolated from the venom of spider Argiope lobata. Argiopine-receptor complex has KD 6,7 .cntdot. 10-7...