EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Isolation of a female-specific, highly repeated Schistosoma mansoni DNA probe and its use in an assay of cercarial sex






Molecular and Biochemical Parasitology, 363: 217-222

Isolation of a female-specific, highly repeated Schistosoma mansoni DNA probe and its use in an assay of cercarial sex

A 476 bp fragment of female-specific Schistosoma mansoni genomic DNA, clone W1, represents a degenerative repeat present in more than 500 copies per female genome, and may be part of the constitutive heterochromatin of the W chromosome. The cloning method described can be used as a general approach for isolating sex-specific, repeated DNA fragments. Using W1 as a probe, we have developed a rapid and accurate dot-blot assay for determining the sex of S. mansoni cercariae.

Accession: 001871957

PMID: 2797060

DOI: 10.1016/0166-6851(89)90169-2

Download PDF Full Text: Isolation of a female-specific, highly repeated Schistosoma mansoni DNA probe and its use in an assay of cercarial sex



Related references

Isolation of a highly repeated w chromosome schistosoma mansoni dna probe and its use in an assay of cercarial sex. Journal of Cellular Biochemistry Supplement (13 PART E): 95, 1989

Highly repeated short DNA sequences in the genome of Schistosoma mansoni recognized by a species-specific probe. Molecular and Biochemical Parasitology 44.1: 73-80, 1991

Infection induces antibodies against the cercarial secretions, but not against the cercarial elastases of Schistosoma mansoni, Schistosoma haematobium, Schistosoma japonicum and Trichobilharzia ocellata. Parasite Immunology 23(10): 557-565, 2001

Isolation of a highly repeated male specific rabbit dna probe for use in in situ hybridization to distinguish male from female cells. American Review of Respiratory Disease 145(4 PART 2): A622, 1992

Isolation of a female specific complementary dna clone from schistosoma mansoni. Journal Of Cellular Biochemistry Supplementrt A: 137, 1986

The host parasite relationship between the saudi arabian schistosoma mansoni and its intermediate and definitive hosts 3. effects of duration of exposure and cercarial concentration on the infection of mice by schistosoma mansoni cercariae. Southeast Asian Journal of Tropical Medicine and Public Health 18(4): 449-452, 1987

Immunocytochemical studies on Schistosoma mansoni. II. Soluble cercarial antigens in cercarial and schistosomules. Journal of Parasitology 62(5): 709-714, 1976

Immunochemical studies on the cercarial-specific calcium binding protein of Schistosoma mansoni. Parasitology 108: 289-300, 1994

Schistosoma mansoni cercarial antigen detection by enzyme immune assay rapid and sensitive contaminated water identification. Abstracts of the Annual Meeting of the American Society for Microbiology 84: ABSTRACT Q31, 1984

Protective capacity of cercarial transformation fluid alone or in combination with crude cercarial antigen against challenge infections of Schistosoma mansoni in mice. Journal of Helminthology (): 1-8, 2016