EurekaMag
+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

Isolation, characterization and partial purification of a transferable membrane channel (amoebapore) produced by Entamoeba histolytica


Isolation, characterization and partial purification of a transferable membrane channel (amoebapore) produced by Entamoeba histolytica



Molecular and Biochemical Parasitology 33(3): 237-247



ISSN/ISBN: 0166-6851

PMID: 2468084

DOI: 10.1016/0166-6851(89)90085-6

Entamoeba histolytica kills cells by contact dependent cytolysis. The mechanism underlying this process must be of rapid onset because target cells round up and show marked zeiosis within 15 min of contact. In earlier work, we identified a remarkable ion-channel forming protein which we named amoebapore, that may contribute to the amoeba-induced target cell killing. Within the amoeba it exists as part of a supramolecular aggregate together with other proteins of unknown function. In this work we report the purification of a solubilized form of the amoebapore. Amoebapore was found to exist as an apparent dimer of the previously reported protein whose molecular weight had been determined under denaturing conditions. Two isoforms of this dimer, with pI values of 6.8 and 5.3 present at a ratio of 7 to 1, were identified and purified. Both isoforms demonstrate ion-channel forming activity in planar lipid membranes. These channels show a unit conductance of 5-20 pS and remain open for less than 1 s. Upon lateral aggregation, opening becomes concerted to a greater degree with channel conductance are observed. The isolated particulate form of amoebapore depolarizes cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001872257

Download citation: RISBibTeXText

Related references

Isolation, purification, and partial characterization of an enterotoxin from extracts of Entamoeba histolytica trophozoites. Infection and Immunity 48(1): 211-218, 1985

Subcellular fractionation of amoebapore and plasma membrane components of Entamoeba histolytica using self-generating Percoll gradients. Molecular and Biochemical Parasitology 14(2): 231-248, 1985

Isolation and partial purification of a surface glycoconjugate of Entamoeba histolytica. Molecular and Biochemical Parasitology 501: 127-138, 1992

Isolation and partial purification of a phosphorylated lipid containing glycoconjugate of entamoeba histolytica. Clinical Research 39(2): 244A, 1991

Partial purification and characterization of Entamoeba histolytica proteinases. Journal of Protozoology 23(2): 21A, 1976

The partial purification and characterization of adenosine kinase from Entamoeba histolytica. American Journal of Tropical Medicine and Hygiene 32(5): 976-979, 1983

Purification and partial characterization of an hemolytic activity from Entamoeba histolytica. Archives of Medical Research 23(2): 243-248, 1992

Partial purification and characterization of ornithine decarboxylase from Entamoeba histolytica. International Journal for Parasitology 26(3): 253-260, 1996

Identification and partial purification of an Entamoeba histolytica membrane protein that binds fibronectin. Archives of Medical Research 23(2): 119-123, 1992

Partial purification and characterization of proteolytic enzymes in various strains of entamoeba histolytica. Molecular and Biochemical Parasitology (Suppl.): 378, 1982

Isolation and partial characterization of a surface glycoconjugate of Entamoeba histolytica. Molecular and Biochemical Parasitology 50(1): 127-138, 1992

Partial purification and characterization of dolichol phosphate mannose synthase from Entamoeba histolytica. Glycobiology 10(12): 1311-1316, 2000

Partial purification and biochemical characterization of a soluble alpha-glucosidase from Entamoeba histolytica. FASEB Journal 15(5): A870, 2001

Isolation and partial characterization of a pore forming protein of pathogenic entamoeba histolytica. American Journal of Tropical Medicine and Hygiene 45(3 Suppl.): 251, 1991

Amoebapore is an important virulence factor of Entamoeba histolytica. Journal of Biosciences Bangalore 27(6): 579-587(Suppl. 3), 2002