EurekaMag.com logo
+ Site Statistics
References:
54,215,046
Abstracts:
30,230,908
PMIDs:
28,215,208
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Kinetics of carrot somatic embryo development in suspension culture



Kinetics of carrot somatic embryo development in suspension culture



Biotechnology and Bioengineering 35(8): 781-786



Somatic embryos in liquid culture can serve as a mass cloning system in a plant propagation program. A quantitative formulation of embryo development obtained from cell suspension cultures is used to develop a segregated kinetic model. The model is based on standard classification schemes as previously developed by plant physiologists. Dependent variables include carbohydrate concentrations (sucrose, fructose, and glucose) and biomass apportioned among the inoculum (free single cells, cell clusters), normal developmental stages, and aberrant cell and embryo types. Good agreement between the model and experimental results is indicated and allows for a rigorous approach to media optimization and reactor scaleup for embryo formation.

(PDF emailed within 0-6 h: $19.90)

Accession: 001873406

Download citation: RISBibTeXText

PMID: 18592578

DOI: 10.1002/bit.260350805



Related references

Effects of light on somatic embryo development and abscisic levels in carrot suspension cultures. Plant Cell Tissue & Organ Culture 11(3): 189-208, 1987

Studies on kinetics of somatic embryo suspension culture in Picea meyeri Rehd. et Wils. Sheng Wu Gong Cheng Xue Bao 16(2): 218-220, 2000

Isolation of putative glycoprotein gene from early somatic embryo of carrot and its possible involvement in somatic embryo development. Plant & Cell Physiology 45(11): 1658-1668, 2004

Static suspension culture of carrot somatic embryos. Journal of Bioscience and Bioengineering 99(3): 300-302, 2005

Synchronization of somatic embryogenesis in a carrot cell suspension culture. Plant Physiology 64(1): 162-164, 1979

A revision of the medium for somatic embryogenesis in carrot suspension culture. Journal of the Faculty of Agriculture Hokkaido University = Hokkaido Daigaku Nogaku bu kiyo 60(3): 183-193, 1981

Frequency improvement of somatic embryogenesis at high embryo density by partial replacement of medium in carrot suspension cultures. Journal of Fermentation & Bioengineering 84(3): 275-278, 1997

Description of somatic-embryo-forming single cells in carrot suspension cultures employing video cell tracking. Planta 94(4): 565-572, 1994

Involvement of endogenous auxin in somatic embryogenesis in a carrot cell suspension culture. Zeitschrift fur Pflanzenphysiologie 95(1): 13-19, 1979

Molecular mechanisms of somatic embryogenesis in a synchronous system in a carrot suspension culture. Proceedings Of The International Botanical Congress:, 1981

Aspects of DNA, RNA and protein synthesis during somatic embryogenesis in a carrot cell suspension culture. Physiologia plantarum 49(2): 255-260, 1980

Mode of action of 2,4-D and zeatin on somatic embryogenesis in a carrot cell suspension culture. Zeitschrift fur Pflanzenphysiologie 99(1): 1-8, 1980

Pyrimidine nucleotide biosynthesis during somatic embryogenesis in a carrot cell suspension culture. Zeitschrift fur Pflanzenphysiologie = International journal of plant physiology04(2): 129-137, 1981

Synchronization of somatic embryogenesis in a carrot daucus carota cell suspension culture. Plant Physiology (Rockville) 64(1): 162-164, 1979

Developmental kinetins of carrot somatic embryo culture. In Vitro Cellular & Developmental Biology Animal 30A(3 PART 2): 33, 1994