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N2O reduction and HD formation by nitrogenase from a nifV mutant of Klebsiella pneumoniae



N2O reduction and HD formation by nitrogenase from a nifV mutant of Klebsiella pneumoniae



Journal of Bacteriology 171(6): 3176-3180



Dinitrogenase from a nifV mutant of Klebsiella pneumoniae contains an altered form of iron-molybdenum cofactor (FeMoco) that lacks a biologically active homocitric acid molecule. Change in the composition of FeMoco led to substantial variation in the kinetics of nitrogenase action. The Kms of the mutant enzyme for N2 and N2O were 0.244 and 0.175 atm (24,714 and 17,726 kPa), respectively. The Km for N2 was higher and that for N2O was lower than that for the wild-type enzyme.

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Accession: 001891654

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PMID: 2656643



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