EurekaMag.com logo
+ Site Statistics
References:
54,215,046
Abstracts:
30,230,908
PMIDs:
28,215,208
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

NADH Induces the Generation of Superoxide Radicals in Leaf Peroxisomes



NADH Induces the Generation of Superoxide Radicals in Leaf Peroxisomes



Plant Physiology 89(3): 728-731



In peroxisomes isolated from pea leaves (Pisum sativum L.) the production of superoxide free radicals (O(2) (-)) by xanthine and NADH was investigated. In peroxisomal membranes, 100 micromolar NADH induced the production of O(2) (-) radicals. In the soluble fractions of peroxisomes, no generation of O(2) (-) radicals was observed by incubation with either NADH or xanthine, although xanthine oxidase was found located predominantly in the matrix of peroxisomes. The failure of xanthine to induce superoxide generation was probably due to the inability to fully suppress the endogenous Mn-superoxide dismutase activity by inhibitors which were inactive against xanthine oxidase. The generation of superoxide radicals in leaf peroxisomes together with the recently described production of these oxygen radicals in glyoxysomes (LM Sandalio, VM Fernández, FL Rupérez, LA del Río [1988] Plant Physiol 87: 1-4) suggests that O(2) (-) generation could be a common metabolic property of peroxisomes and further supports the existence of active oxygen-related rôles for peroxisomes in cellular metabolism.

(PDF emailed within 0-6 h: $19.90)

Accession: 001891660

Download citation: RISBibTeXText

PMID: 16666612

DOI: 10.1104/pp.89.3.728



Related references

Characterization of membrane polypeptides from pea leaf peroxisomes involved in superoxide radical generation. Biochemical Journal 337: 531-536, 1999

New insights into the superoxide generation sites in bovine heart NADH-ubiquinone oxidoreductase (Complex I): the significance of protein-associated ubiquinone and the dynamic shifting of generation sites between semiflavin and semiquinone radicals. Biochimica et Biophysica Acta 1797(12): 1901-1909, 2011

Superoxide generation from mitochondrial NADH dehydrogenase induces self-inactivation with specific protein radical formation. Journal of Biological Chemistry 280(45): 37339-37348, 2005

Avidin attachment to biotinylated human neutrophils induces generation of superoxide radicals. Biologicheskie Membrany (Moscow) 9(12): 1211-1221, 1992

Intraorganellar distribution of superoxide dismutase in plant peroxisomes (glyoxysomes and leaf peroxisomes). Plant Physiology 88(4): 1215-1218, 1988

Oxygen radicals shaping evolution: why fatty acid catabolism leads to peroxisomes while neurons do without it: FADH₂/NADH flux ratios determining mitochondrial radical formation were crucial for the eukaryotic invention of peroxisomes and catabolic tissue differentiation. Bioessays 33(2): 88-94, 2011

Cadmium induces interleukin-8 production in human peripheral blood mononuclear cells with the concomitant generation of superoxide radicals. Lymphokine and Cytokine Research 12(6): 421-428, 1993

Generation of superoxide anion radicals and hydroxyl radicals in chloroplast thylakoids. Asada, K, Yoshikawa, T International Congress Series; Frontiers of reactive oxygen species in biology and medicine 31-32, 1994

Generation of superoxide radicals and activity of superoxide dismutase in the blood of patients with cerebral dyshemias. Zhurnal Eksperimental'noi i Klinicheskoi Meditsiny 27(4): 364-368, 1987

Generation of superoxide radicals in alkaline solutions of hydrogen peroxide and the effect of superoxide dismutase on this system. Biochimica et Biophysica Acta 583(3): 279-286, 1979

Changes of superoxide anion radicals and superoxide dismutase activity during senescence of flag leaf in rice. Chinese Journal of Rice Science 7(1): 51-54, 1993

Methyl 4 phenylpyridinium mpp induces nadh dependent superoxide formation and enhances nadh dependent lipid peroxidation in bovine heart submitochondrial particles. Biochemical & Biophysical Research Communications 170(3): 1049-1055, 1990

NADH- and NADPH-dependent formation of superoxide radicals in liver nuclei. Biokhimiia 54(6): 1020-1025, 1989

Effects of scavengers of superoxide radicals, hydrogen peroxide, singlet oxygen and hydroxyl radicals on malondialdehyde generation from arachidonic acid by bovine seminal vesicle microsomes. Experientia 36(12): 1339-1340, 1980