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Production and characterization of monoclonal antibodies against Actinobacillus (Haemophilus) pleuropneumoniae serotype 2



Production and characterization of monoclonal antibodies against Actinobacillus (Haemophilus) pleuropneumoniae serotype 2



Nihon Juigaku Zasshi. Japanese Journal of Veterinary Science 52(3): 533-542



Four hybridoma cell lines producing monoclonal antibodies (MAbs) against A. pleuropneumoniae were established by fusion of mouse myeloma and spleen cells obtained from mice immunized with a serotype 2, strain SH-15. ELISA-inhibition tests with antigens obtained from 12 serotype strains of A. pleuropneumoniae and 9 other gram-negative bacteria showed that all the MAbs bound to only serotype 2 strains of A. pleuropneumoniae.

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Accession: 001919749

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PMID: 2385034

DOI: 10.1292/jvms1939.52.533


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