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Purification and characterization of eclosion hormone from the moth, Manduca sexta



Purification and characterization of eclosion hormone from the moth, Manduca sexta



Insect Biochemistry, 187: 701-707



The eclosion hormone (EH) of the sphingid Manduca sexta is a 62 amino acid peptide which causes the behavioural and physiological changes that occur at ecdysis. EH was purified from the corpora cardiaca-corpora allata (CC-CA) complexes from pharate adults using acidic methanol extraction followed by 2 high performance liquid chromatography separation steps. One CC-CA complex was found to store about 3.5 ng of EH.

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Accession: 001923617

Download citation: RISBibTeXText

DOI: 10.1016/0020-1790(88)90079-0



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