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Purification of the Drosophila Kc cell juvenile hormone binding protein



Purification of the Drosophila Kc cell juvenile hormone binding protein



Insect Biochemistry, 193: 327-335



The juvenile hormone binding protein (JHBP) from the cytosol of cells of the Kc line of Drosophila melanogaster was purified with the use of a juvenile hormone photoaffinity analogue, 10,11-epoxy(2E,6E)farnesyl diazoacetate (EFDA). The purification procedure consisted of 5 chromatographic steps and the end product of the purification procedure showed homogeneity by means of both native and SDS polyacrylamide gel electrophoresis.

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Accession: 001923881

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DOI: 10.1016/0020-1790(89)90079-6


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