Responses of guard cell protoplasts of Nicotiana glauca from leaves with closed or open stomata to red and blue light

Marsh, P.; Dodge, S.; Tallman, G.

In experiments with guard cell protoplasts (GCP) from Nicotiana glauca, rates of red light-induced alkaninization and blue light-induced acidification varied with the time of day that the GCP were isolated. Conductances of single leaves were measured either pre-dawn or at mid-morning, and GCP were isolated from their abaxial epidermes. GCP from both leaf types hydrolyzed fluorescein diacetate and swelled to equal diam. in solutions containing KCl and fusicoccin.