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Scaling-up of a batch whey fermentation by Kluyveromyces fragilis

Scaling-up of a batch whey fermentation by Kluyveromyces fragilis

Applied microbiology and biotechnology 31(5-6): 495-501

A whey fermentation by Kluyveromyces fragilis was scaled-up to a 1000-dm3 stirred fermentor, by varying the stirrer speed, the air-flow rate and the initial concentration of lactose. Its evolution was simulated by applying the same unstructured model (consisting of a microbial specific growth rate of pseudo-first order with respect to the COD concentration and constant biomass yield per unit COD removed) set up in previous experiments using 8- to 80-dm3 fermentors. Despite the great scale-up ratios, very different operating conditions, and geometric dissimilarity, a series of empirical regressions previously developed allowed approximate, but acceptable prediction of the stoichiometric and kinetic coefficients of the above mathematical model, thus confirming the capacity of this model to provide a reliable basis for further scale-up of this fermentation process to a production scale.

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Accession: 001939512

Download citation: RISBibTeXText

DOI: 10.1007/bf00270783

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