EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Screening antenatal blood samples for anti-human immunodeficiency virus antibodies by a large-pool enzyme-linked immunosorbent assay system






South African Medical Journal 76(6): 245-247

Screening antenatal blood samples for anti-human immunodeficiency virus antibodies by a large-pool enzyme-linked immunosorbent assay system

Blood collected over an 18-month period in 1987-88 from 104 683 pregnant women resident in the southern Transvaal was tested in pools for HIV antibodies. For the initial screening 48 samples (5 micro l) of plasma were pooled, diluted, and tested by ELISA (Organon-Teknika). Positive pools were further analysed until repeatedly positive individual reactions were identified and tested with confirmatory tests.


Accession: 001940052



Related references

Shapiro, M.; Crookes, R.L.; O'Sullivan, E., 1989: Screening antenatal blood samples for anti-human immunodeficiency virus antibodies by a large-pool enzyme-linked immunosorbent assay system. Results of an 18-month investigation. Blood samples submitted for routine antenatal testing from 104,683 pregnant women resident in the southern Transvaal were screened for antibodies to the human immunodeficiency virus (HIV) during an 18-month period--1 May 1987 - 31 October 1988. Al...

Shapiro M.; Crookes R.L.; O'sullivan E., 1989: Screening antenatal blood samples for anti human immunodeficiency virus antibodies by a large pool elisa system results of an 18 month investigation. Blood samples submitted for routine antenatal testing from 104 683 pregnant women resident in the southern Transvaal were screened for antibodies to the human immunodeficiency virus (HIV) during an 18-month period - 1 May 1987 - 31 October 1988. A...

Novack, L.; Galai, N.; Yaari, A.; Orgel, M.; Shinar, E.; Sarov, B., 2006: Use of seroconversion panels to estimate delay in detection of anti-human immunodeficiency virus antibodies by enzyme-linked immunosorbent assay of pooled compared to singleton serum samples. The transfusion of unsafe blood worldwide accounts for 5 to 15% of new human immunodeficiency virus (HIV) infections, most of which occur in sub-Saharan Africa. While developed countries now apply PCR testing of pooled samples, some developing cou...

Thongcharoen, P.; Wasi, C.; Louisirirotchanakul, S.; Parry, J.; Connell, J.; Mortimer, P., 1992: Immunoglobulin G antibody capture enzyme-linked immunosorbent assay: a versatile assay for detection of anti-human immunodeficiency virus type 1 and 2 antibodies in body fluids. In tests on specimens of dried blood, saliva, and urine from 55 human immunodeficiency virus (HIV)-seropositive and 55 HIV-seronegative patients, an immunoglobulin G capture enzyme immunoassay for the detection of antibodies to HIV types 1 and 2,...

Bhimji, A.; Zaragoza, A.A.; Live, L.S.; Kelley, S.O., 2014: Electrochemical enzyme-linked immunosorbent assay featuring proximal reagent generation: detection of human immunodeficiency virus antibodies in clinical samples. We describe a simple electrochemical immunoassay for human immunodeficiency virus (HIV) antibody detection that localizes capture and detection reagents in close proximity to a microelectrode. Antigenic peptides from HIV-1 gp41 or HIV-2 gp36 were...

Zeng, P.; Liu, J.; Wang, J.; Dong, X.; Li, J.; Bi, X.; Ma, H.; Wen, X.; He, M.; Liu, Y.; Ness, P.; Shan, H., 2016: Parallel enzyme-linked immunosorbent assay screening for human immunodeficiency virus among blood donors in five Chinese blood centres: a retrospective analysis. To evaluate the strategy of parallel screening with different enzyme-linked immunosorbent assays (ELISA) among Chinese blood donors. Parallel screening with ELISA has been the main strategy to detect human immunodeficiency virus (HIV) in blood don...

Kannangai, R.; Ramalingam, S.; Prakash, K.J.; Abraham, O.C.; George, R.; Castillo, R.C.; Schwartz, D.H.; Jesudason, M.V.; Sridharan, G., 2001: A peptide enzyme linked immunosorbent assay (ELISA) for the detection of human immunodeficiency virus type-2 (HIV-2) antibodies: an evaluation on polymerase chain reaction (PCR) confirmed samples. HIV-1 and HIV-2 infections differ in prognosis, and may also require different prevention and/or treatment approaches. Thus, estimating the true prevalence of HIV-1 and HIV-2 infections, as well as co-infections, is a critical step in controlling...

Ozanne, G.; Fauvel, M., 1988: Performance and reliability of five commercial enzyme-linked immunosorbent assay kits in screening for anti-human immunodeficiency virus antibody in high-risk subjects. Anti-human immunodeficiency virus enzyme-linked immunosorbent assay kits marketed by Electro-Nucleonics Inc. (ENI), Genetic Systems Corp. (GSC), Organon Teknika Inc. (OTI), Ortho Diagnostic Systems Inc. (ODSI), and Wellcome Diagnostics (WD) were e...

Kobayashi, S.; Yoshida, T.; Hiroshige, Y.; Matsui, T.; Yamamoto, N., 1988: Comparative studies of commercially available particle agglutination assay and enzyme-linked immunosorbent assay for screening of human T-cell leukemia virus type I antibodies in blood donors. Human T-cell leukemia virus type I (HTLV-I) transmission during blood transfusion can be prevented by screening for and eliminating blood containing anti-HTLV-I antibodies. For this purpose, we evaluated two commercial test kits for HTLV-I antibod...

Furuya, T.; Hasegawa, A.; Miyazawa, T.; Miki, K.; Mikami, T., 1992: Detection of anti-gag antibodies of feline immunodeficiency virus in cat sera by enzyme-linked immunosorbent assay. Using gag protein of feline immunodeficiency virus (FIV) expressed in Escherichia coli, an enzyme-linked immunosorbent assay (ELISA) system was developed for detection of antibodies to FIV gag protein in cat sera. With serum samples from cats expe...