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Screening antenatal blood samples for anti-human immunodeficiency virus antibodies by a large-pool enzyme-linked immunosorbent assay system






South African Medical Journal 76(6): 245-247

Screening antenatal blood samples for anti-human immunodeficiency virus antibodies by a large-pool enzyme-linked immunosorbent assay system

Blood collected over an 18-month period in 1987-88 from 104 683 pregnant women resident in the southern Transvaal was tested in pools for HIV antibodies. For the initial screening 48 samples (5 micro l) of plasma were pooled, diluted, and tested by ELISA (Organon-Teknika). Positive pools were further analysed until repeatedly positive individual reactions were identified and tested with confirmatory tests.

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Accession: 001940052



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