Sequence of the gene encoding the mitochondrial F1-ATPase alpha subunit from Nicotiana plumbaginifolia
Chaumont, F.; Boutry, M.; Briquet, M.; Vassarotti, A.
Nucleic Acids Research 16(13): 6247
A genomic bank of N. plumbaginifolia mitochondrial DNA was constructed and probed with the gene encoding the alpha -subunit of maize mitochondrial F1-ATPase. A 4.7 kb insert was present in the positive clones isolated. After subcloning, a series of progressive deletions were generated. Sequence analysis (presented) revealed a region of 1527 nucleotides encoding a polypeptide of 55 162 Da.