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Sequence of the gene encoding the mitochondrial F1-ATPase alpha subunit from Nicotiana plumbaginifolia

Chaumont, F.; Boutry, M.; Briquet, M.; Vassarotti, A.

Nucleic Acids Research 16(13): 6247

1988


ISSN/ISBN: 0305-1048
PMID: 2899872
DOI: 10.1093/nar/16.13.6247
Accession: 001943193

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A genomic bank of N. plumbaginifolia mitochondrial DNA was constructed and probed with the gene encoding the alpha -subunit of maize mitochondrial F1-ATPase. A 4.7 kb insert was present in the positive clones isolated. After subcloning, a series of progressive deletions were generated. Sequence analysis (presented) revealed a region of 1527 nucleotides encoding a polypeptide of 55 162 Da.

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