Section 2
Chapter 1,949

Somatic hybridization in Citrus: navel orange (C. sinensis Osb.) and grapefruit (C. paradisi Macf.)

Ohgawara, T.; Kobayashi, S.; Ishii, S.; Yoshinaga, K.; Oiyama, I.

Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 78(5): 609-612


ISSN/ISBN: 0040-5752
PMID: 24225818
DOI: 10.1007/bf00262553
Accession: 001948515

Protoplasts of navel orange, isolated from embryogenic nucellar cell suspension culture, were fused with protoplasts of grapefruit isolated from leaf tissue. The fusion products were cultured in hormone-free medium containing 0.6M sucrose. Under the culture conditions, somatic embryogenesis of navel orange protoplasts was suppressed, while cell division of grapefruit mesophyll protoplasts was not induced. Six embryoids were obtained.

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