Structure and expression of the U5 snRNA gene of Arabidopsis thaliana. Conserved upstream sequence elements in plant U-RNA genes

Vankan, P.; Edoh, D.; Filipowicz, W.

Nucleic Acids Research 16(22): 10425-10440


ISSN/ISBN: 0305-1048
PMID: 3205713
DOI: 10.1093/nar/16.22.10425
Accession: 001954380

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The gene encoding a 125 nucleotide U5 sn (small nuclear) RNA of Arabidopsis was isolated. Activity of the cloned gene was demonstrated in stably transformed tobacco calluses and by transient expression in transfected protoplasts of Nicotiana plumbaginifolia. Southern analysis indicated that the Arabidopsis genome contains 8-9 copies of the U5 gene. Alignment of upstream non-coding regions revealed 2 elements conserved between all plant U-RNA genes characterized so far: the sequence RTCCCACATCG (-70/-80 region, 100% conservation) and the TATA homology around position -30. The coding regions in all genes are followed by the sequence CAN4-9AGTN(A/T)AA which may correspond to a termination and/or processing signal.