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Synergistic effect of insulin and follicle-stimulating hormone on biochemical and morphological differentiation of porcine granulosa cells in vitro


, : Synergistic effect of insulin and follicle-stimulating hormone on biochemical and morphological differentiation of porcine granulosa cells in vitro. Biology of Reproduction 39(2): 379-390

Porcine granulosa cells isolated from small antral follicles (controls) cultured in the absence of exogenous hormones exhibited few microvilli and gap junctions; the mitochondria, endoplasmic reticulum, and Golgi complex were all poorly developed. Progesterone secretion was negligible and the cells bound little odo-HCG.

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Accession: 001962322

PMID: 3140905

DOI: 10.1095/biolreprod39.2.379

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Amsterdam A.; May J.V.; Schomberg D.W., 1988: Synergistic effect of insulin and fsh on biochemical and morphological differentiation of porcine granulosa cells in vitro. Insulin and follicle-stimulating hormone (FSH) have been shown to facilitate granulosa cell differentiation in vitro. To gain insight into this process, we evaluated the effects of these hormones, alone and in combination, upon the biochemical par...

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