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Synergistic effects of ketoconazole and SF-86327 on the proliferation of epimastigotes and amastigotes of Trypanosoma (Schizotrypanum) cruzi






Annals of the New York Academy of Sciences 544: 357-358

Synergistic effects of ketoconazole and SF-86327 on the proliferation of epimastigotes and amastigotes of Trypanosoma (Schizotrypanum) cruzi

Proliferation of epimastigotes of T. cruzi in LIT medium at 28 degrees C was immediately arrested by ketoconazole or SF-86327 at concentrations 3 x 10-5 M. SF-86327 stopped cell growth at 10-6 M after 48 h and ketoconazole at 3 x 10-6 M stopped cell growth after 120 h. The concentration of SF-86327 required to reduce cell growth to 25% of controls in 144 h was reduced 33-fold in the presence of 10-6 M ketoconazole (which on its own reduced growth by only 30%). SF-86327 at 10-7 M reduced the number of VERO cells infected with amastigotes to 50% of controls, and at 3 x 10-6 M completely eradicated the parasite. Corresponding figures for ketoconazole were 10-10 M and 10-8 M. The concentration of SF-86327 required to reduce the number of infected cells to 50% of controls was 100-fold lower in the presence of ketoconazole at 10-11 M (which by itself had no effect on amastigote proliferation). Complete eradication of the parasite was obtained when using the drugs in combination at concentrations as low as 10-9 M.

Accession: 001962336

PMID: 3063176

DOI: 10.1111/j.1749-6632.1988.tb40421.x

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