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Synergistic stimulation of prostaglanding E2 production by calcium ionophore and protein kinase C activator in rat granulosa cells

, : Synergistic stimulation of prostaglanding E2 production by calcium ionophore and protein kinase C activator in rat granulosa cells. Biology of Reproduction 40(5): 1000-1006

The effects of LHRH and its putative intracellular mediators on progesterone (P) and PGE2 formation were studied in rat granulosa cells. A calcium ionophore (A23187), 12-O-tetradecanoylphorbol-13-acetate (TPA), and melittin (a phospholipase A2-stimulator) were used to enhance intracellular calcium, protein kinase C and arachidonic acid concentrations resp.

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