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Synthesis and processing of kinetoplast DNA minicircles in Trypanosoma equiperdum


Molecular and Cellular Biology 9(8): 3212-3217
Synthesis and processing of kinetoplast DNA minicircles in Trypanosoma equiperdum
Studies on the synthesis and processing of the minicircle L and H strands of the kinetoplast DNA of T. equiperdum are reported. Analysis of free minicircles by two-dimensional agarose gel electrophoresis indicated that elongating L strands are present on theta structures. Hybridization studies indicated that L-strand elongation is continuous and unidirectional, starting near nucleotide 805 and proceeding around the entire minicircle.


Accession: 001962490

PMID: 2552285

DOI: 10.1128/mcb.9.8.3212



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