EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Synthesis and processing of the transmembrane envelope protein of equine infectious anemia virus


, : Synthesis and processing of the transmembrane envelope protein of equine infectious anemia virus. Journal of Virology 64(8): 3770-3778

The transmembrane (TM) envelope protein of lentiviruses, including equine infectious anemia virus (EIAV), is significantly larger than that of other retroviruses and may extend in the C-terminal direction 100 to 200 amino acids beyond the TM domain. This size difference suggests a lentivirus-specific function for the long C-terminal extension. We have investigated the synthesis and processing of the EIAV TM protein by immune precipitation and immunoblotting experiments, by using several envelope-specific peptide antisera. We show that the TM protein in EIAV particles is cleaved by proteolysis to an N-terminal glycosylated 32- to 35-kilodalton (kDa) segment and a C-terminal nonglycosylated 20-kDa segment. The 20-kDa fragment was isolated from virus fractionated by high-pressure liquid chromatography, and its N-terminal amino acid sequence was determined for 13 residues. Together with the known nucleotide sequence, this fixes the cleavage site at a His-Leu bond located 240 amino acids from the N terminus of the TM protein. Since the 32- to 35-kDa fragment and the 20-kDa fragment are not detectable in infected cells, we assume that cleavage occurs in the virus particle and that the viral protease may be responsible. We have also found that some cells producing a tissue-culture-adapted strain of EIAV synthesize a truncated envelope precursor polyprotein. The point of truncation differs slightly in the two cases we have observed but lies just downstream from the membrane-spanning domain, close to the cleavage point described above. In one case, virus producing the truncated envelope protein appeared to be much more infectious than virus producing the full-size protein, suggesting that host cell factors can select for virus on the basis of the C-terminal domain of the TM protein.


Accession: 001962492

PMID: 2164597

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Chong, Y.H.; Ball, J.M.; Issel, C.J.; Montelaro, R.C.; Rushlow, K.E., 1991: Analysis of equine humoral immune responses to the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus. Defined segments of the transmembrane envelope glycoprotein (gp45) of equine infectious anemia virus were expressed as TrpLE fusion proteins and examined for their reactivity in Western immunoblots against a diverse panel of equine immune sera. Th...

Yin, X.; Hu, Z.; Gu, Q.; Wu, X.; Zheng, Y-Hui.; Wei, P.; Wang, X., 2014: Equine tetherin blocks retrovirus release and its activity is antagonized by equine infectious anemia virus envelope protein. Human tetherin is a host restriction factor that inhibits replication of enveloped viruses by blocking viral release. Tetherin has an unusual topology that includes an N-terminal cytoplasmic tail, a single transmembrane domain, an extracellular do...

McGuire, T.C.; Leib, S.R.; Mealey, R.H.; Fraser, D.G.; Prieur, D.J., 2003: Presentation and binding affinity of equine infectious anemia virus CTL envelope and matrix protein epitopes by an expressed equine classical MHC class I molecule. Control of a naturally occurring lentivirus, equine infectious anemia virus (EIAV), occurs in most infected horses and involves MHC class I-restricted, virus-specific CTL. Two minimal 12-aa epitopes, Env-RW12 and Gag-GW12, were evaluated for prese...

Sun, C.; Zhang, B.; Jin, J.; Montelaro, R.C., 2008: Binding of equine infectious anemia virus to the equine lentivirus receptor-1 is mediated by complex discontinuous sequences in the viral envelope gp90 protein. The identification and characterization of a functional cellular receptor for equine infectious anemia virus (EIAV), designated equine lentivirus receptor-1 (ELR1), a member of the tumour necrosis factor receptor protein family, has been reported...

Thomas, L.M.; Huntington, P.J.; Mead, L.J.; Wingate, D.L.; Rogerson, B.A.; Lew, A.M., 1992: A soluble recombinant fusion protein of the transmembrane envelope protein of equine infectious anaemia virus for ELISA. The use of the bacterial expression vector, pGex, to produce an abundant, soluble fusion protein of gp45 from equine infectious anaemia virus is described. Purification of the recombinant protein was achieved by one step affinity chromatography on...

Yuan, Q.; Liu, C.; Liang, Z.; Chen, X.; Diao, D.; Kong, X., 2013: The comparison of genetic variation in the envelope protein between various immunodeficiency viruses and equine infectious anemia virus. The envelope protein (Env) of lentiviruses such as HIV, SIV, FIV and EIAV is larger than that of other retroviruses. The Chinese EIAV attenuated vaccine is based on Env and has helped to successfully control this virus, demonstrating that envelope...

Anonymous, 2012: Recombinant envelope protein rgp9 ELISA for equine infectious anemia virus provides comparable results to the agar gel immunodiffusion. Equine infectious anemia (EIA) is an important viral infection affecting horses worldwide. The course of infection is accompanied generally by three characteristic stages: acute, chronic and inapparent. There is no effective EIA vaccine or treatme...

Reis, J.K.P.; Diniz, R.S.; Haddad, Jão.P.A.; Ferraz, I.B.F.; Carvalho, A.F.; Kroon, E.G.; Ferreira, P.C.P.; Leite, Rômulo.C., 2012: Recombinant envelope protein (rgp90) ELISA for equine infectious anemia virus provides comparable results to the agar gel immunodiffusion. Equine infectious anemia (EIA) is an important viral infection affecting horses worldwide. The course of infection is accompanied generally by three characteristic stages: acute, chronic and inapparent. There is no effective EIA vaccine or treatme...

Wang, X.; Wang, S.; Lin, Y.; Jiang, C.; Ma, J.; Zhao, L.; Lv, X.; Wang, F.; Shen, R.; Zhou, J., 2011: Unique evolution characteristics of the envelope protein of EIAV(LN₄₀), a virulent strain of equine infectious anemia virus. The Chinese equine infectious anemia virus (EIAV) virulent strain EIAV(LN40) is derived from a naturally occurring virus by continuously passing in horses for 16 generations. Its genome sequence is 23% different from that of the American strains o...

Perry, S.T.; Flaherty, M.T.; Kelley, M.J.; Clabough, D.L.; Tronick, S.R.; Coggins, L.; Whetter, L.; Lengel, C.R.; Fuller, F., 1992: The surface envelope protein gene region of equine infectious anemia virus is not an important determinant of tropism in vitro. Virulent, wild-type equine infectious anemia virus (EIAV) is restricted in one or more early steps in replication in equine skin fibroblast cells compared with cell culture-adapted virus, which is fully competent for replication in this cell type....