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Synthesis of gamma -glutamyltaurine by gamma -glutamyltranspeptidase of Penicillium roqueforti

, : Synthesis of gamma -glutamyltaurine by gamma -glutamyltranspeptidase of Penicillium roqueforti. Agricultural and Biological Chemistry 53(12): 3239-3244

The formation of gamma -glutamyltaurine ( gamma -GluTau) from L-glutamine and taurine was carried out with gamma -glutamyltranspeptidase (GGT) of Penicillium roqueforti (IFO 4622). For GGT preparation, a wheat bran koji extract prepared with P. roqueforti (IFO 4622) was used. 180 mM gamma -GluTau was formed, with a yield of 36%, in a reaction mixture comprising 500 mM L-glutamine, 500 mM taurine, 100 mM Tris-HCl buffer (pH 8.5) and 0.6 units of GGT per ml.

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