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Synthesis of infectious RNA from full-length cloned cDNA to RNA of cymbidium ringspot tombusvirus


Journal of General Virology 71: 1857-1860
Synthesis of infectious RNA from full-length cloned cDNA to RNA of cymbidium ringspot tombusvirus
A full-length DNA copy of CRSV RNA was cloned downstream of a phage T7 promoter. In vitro transcripts had no extra nucleotides at the 3' terminus, and a 5' end likely to be precisely as in genomic RNA. Transcripts were infective when inoculated into test plants. Northern blots from inoculated plants revealed the presence of genomic and subgenomic RNAs, but not a satellite RNA.


Accession: 001962579

PMID: 1697330

DOI: 10.1099/0022-1317-71-8-1857



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