EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Synthesis of interleukin-1 (IL-1) by human monocytes cultured in vitro with amphotericin B (AMB)


, : Synthesis of interleukin-1 (IL-1) by human monocytes cultured in vitro with amphotericin B (AMB). Journal of Leukocyte Biology 44(4): 285

To determine whether AmB induces production of IL-1, a potent stimulus for prostaglandin (PG) release, 1 million plastic-adherent mononuclear cells were cultured from normal volunteers for 20 h in dilutions of AmB or lipopolysaccharide (LPS) in RPMI. IL-1 content of supernatant fluids was assessed in a comitogenesis assay using D10 cells. Cells cultured with up to 2.5 micro g/ml AmB or 10 micro g/ml LPS released as much as 200 U IL-1 in a dose-related manner.


Accession: 001962582

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Kaplan, G., 1983: In vitro differentiation of human monocytes. Monocytes cultured on glass are cytotoxic to tumor cells but monocytes cultured on collagen are not. Cultivation of human blood monocytes on glass gives rise to cells nonspecifically cytotoxic to tumor cells. If the monocytes are cultured on collagen gels with no contact with glass, no such cytotoxic activity is induced. Killing appeared to be ex...

Austgulen, R.; Hammerstrøm, J.; Nissen-Meyer, J., 1987: In vitro cultured human monocytes release fibroblast proliferation factor(s) different from interleukin 1. Human monocytes cultured in vitro released factors with growth stimulatory effect upon human dermal fibroblasts. Monocytes in RPM1 1640 released a growth enhancing activity in nearly constant amounts during in vitro differentiation to macrophage-l...

Chalyĭ, I.V.; Kolesnikova, T.S.; Fegeding, K.V.; Voĭtenok, N.N., 1996: Effect of tumor necrosis factor-alpha on synthesis of interleukin-8 by human monocytes and lymphocytes in vitro. Biulleten' Eksperimental'noi Biologii i Meditsiny 122(8): 200-202

Gauldie, J.; Sauder, D.N.; McAdam, K.P.; Dinarello, C.A., 1987: Purified interleukin-1 (IL-1) from human monocytes stimulates acute-phase protein synthesis by rodent hepatocytes in vitro. A universal component of inflammation is the increased synthesis of a series of plasma proteins (acute-phase proteins) by the liver. The postulated messenger of acute-phase protein induction is released by leucocytes at the site of inflammation an...

Mccloy, M.P.; Roberts, I.A.G.; Howarth, L.; Paget, R.; Watts, T.L.; Murray, N.A., 2001: Interleukin-11 downregulates interleukin-1beta but not TNF-alpha or interleukin-6 production by human neonatal monocytes in vitro and in vivo. We have shown that interleukin-11 (IL-11) stimulates neonatal megakaryocytes and that plasma IL-11, undetectable in healthy neonates, is increased in some babies with sepsis-associated thrombocytopenia. In animal studies IL-11 reduces sepsis-assoc...

Morioka, T.; Narita, I.; Shimizu, F.; Oite, T., 1991: Production by cultured human monocytes of mesangial cell proliferation factor(s) differing from interleukin-1 and interleukin-6. Conditioned media from human peripheral blood leucocytes treated with lipopolysaccharide (LPS) induced a marked increase in the 3H-thymidine incorporation of cultured mesangial cells at low serum concentration (four to six times higher than contro...

Postlethwaite A.E.; Smith G.N.Jr; Lachman L.B.; Endres R.O.; Poppleton H.M.; Hasty K.A.; Seyer J.M.; Kang A.H., 1989: Stimulation of glycosaminoglycan synthesis in cultured human dermal fibroblasts by interleukin 1 induction of hyaluronic acid synthesis by natural and recombinant interleukin 1s and synthetic interleukin 1 beta peptide 163 171. Hyaluronic acid (HA) is believed to play a critical role in wound healing and in morphogenesis. Factors controlling the production of HA by fibroblasts in normal and pathological states are not completely understood. In this report we have observe...

Postlethwaite, A.E.; Smith, G.N.; Lachman, L.B.; Endres, R.O.; Poppleton, H.M.; Hasty, K.A.; Seyer, J.M.; Kang, A.H., 1989: Stimulation of glycosaminoglycan synthesis in cultured human dermal fibroblasts by interleukin 1. Induction of hyaluronic acid synthesis by natural and recombinant interleukin 1s and synthetic interleukin 1 beta peptide 163-171. Hyaluronic acid (HA) is believed to play a critical role in wound healing and in morphogenesis. Factors controlling the production of HA by fibroblasts in normal and pathological states are not completely understood. In this report we have observe...

de Waal Malefyt, R.; Abrams, J.; Bennett, B.; Figdor, C.G.; de Vries, J.E., 1991: Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes. In the present study we demonstrate that human monocytes activated by lipopolysaccharides (LPS) were able to produce high levels of interleukin 10 (IL-10), previously designated cytokine synthesis inhibitory factor (CSIF), in a dose dependent fash...

Suzuki M.; Cooper E.L.; Hsu T.; Sarafian S., 1992: Human interleukin 1 promotes dna synthesis in earthworm coelomocytes cultured in vitro. FASEB Journal 6(5): A2055