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Synthesis of interleukin-1 (IL-1) by human monocytes cultured in vitro with amphotericin B (AMB)


, : Synthesis of interleukin-1 (IL-1) by human monocytes cultured in vitro with amphotericin B (AMB). Journal of Leukocyte Biology 44(4): 285

To determine whether AmB induces production of IL-1, a potent stimulus for prostaglandin (PG) release, 1 million plastic-adherent mononuclear cells were cultured from normal volunteers for 20 h in dilutions of AmB or lipopolysaccharide (LPS) in RPMI. IL-1 content of supernatant fluids was assessed in a comitogenesis assay using D10 cells. Cells cultured with up to 2.5 micro g/ml AmB or 10 micro g/ml LPS released as much as 200 U IL-1 in a dose-related manner.

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