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Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris


, : Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 78(3): 445-455

Fusion of leaf protoplasts from an inbred line of Brassica oleracea subsp. botrytis (cauliflower, n = 9), carrying the Ogura (R1) male-sterile cytoplasm, with hypocotyl protoplasts of B. campestris subsp. oleifera (cv. Candle, n = 10), carrying an atrazine-resistant (ATR) cytoplasm, resulted in the production of synthetic B. napus (n = 19). Thirty-four somatic hybrids were produced; they were characterized for morphology, phosphoglucose isomerase [glucose-6-phosphate isomerase] isoenzymes, ribosomal DNA hybridization patterns, chromosome numbers and organelle composition. All somatic hybrids carried atrazine-resistant chloroplasts derived from B. campestris. When the mitochondrial genomes in 19 hybrids were examined by restriction endonuclease and Southern blot analyses, 12 contained mitochondria showing novel DNA restriciton patterns; of these, 5 were male-sterile and 7 were male-fertile. The remaining hybrids contained mitochondrial DNA that was identical to that of the ATR parent and all were male fertile.

Accession: 001962588

PMID: 24227255

DOI: 10.1007/BF00265310

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Jourdan P.S.; Earle E.D.; Mutschler M.A., 1989: Synthesis of male sterile triazine resistant brassica napus by somatic hybridization between cytoplasmic male sterile brassica oleracea and atrazine resistant brassica campestris. Fusion of leaf protoplasts from an inbred line of Brassica oleracea ssp. botrytis (cauliflower, n = 9) carrying the Ogura (R1) male sterile cytoplasm with hypocotyl protoplasts of B. campestris ssp. oleifera (cv "Candle",n = 10) carrying...

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