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Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris



Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris



Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 78(3): 445-455



Fusion of leaf protoplasts from an inbred line of Brassica oleracea subsp. botrytis (cauliflower, n = 9), carrying the Ogura (R1) male-sterile cytoplasm, with hypocotyl protoplasts of B. campestris subsp. oleifera (cv. Candle, n = 10), carrying an atrazine-resistant (ATR) cytoplasm, resulted in the production of synthetic B. napus (n = 19). Thirty-four somatic hybrids were produced; they were characterized for morphology, phosphoglucose isomerase [glucose-6-phosphate isomerase] isoenzymes, ribosomal DNA hybridization patterns, chromosome numbers and organelle composition. All somatic hybrids carried atrazine-resistant chloroplasts derived from B. campestris. When the mitochondrial genomes in 19 hybrids were examined by restriction endonuclease and Southern blot analyses, 12 contained mitochondria showing novel DNA restriciton patterns; of these, 5 were male-sterile and 7 were male-fertile. The remaining hybrids contained mitochondrial DNA that was identical to that of the ATR parent and all were male fertile.

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Accession: 001962588

Download citation: RISBibTeXText

PMID: 24227255

DOI: 10.1007/BF00265310



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