+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris



Synthesis of male sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male sterile B. oleracea and atrazine-resistant B. campestris



Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 78(3): 445-455



Fusion of leaf protoplasts from an inbred line of Brassica oleracea ssp. botrytis (cauliflower, n=9) carrying the Ogura (R1) male sterile cytoplasm with hypocotyl protoplasts of B. campestris ssp. oleifera (cv "Candle", n=10) carrying an atrazine-resistant (ATR) cytoplasm resulted in the production of synthetic B. napus (n=19). Thirty-four somatic hybrids were produced; they were characterized for morphology, phosphoglucose isomerase isoenzymes, ribosomal DNA hybridization patterns, chromosome numbers, and organelle composition. All somatic hybrids carried atrazine-resistant chloroplasts derived from B. campestris. The mitochondrial genomes in 19 hybrids were examined by restriction endonuclease and Southern blot analyses. Twelve of the 19 hybrids contained mitochondria showing novel DNA restriction patterns; of these 12 hybrids, 5 were male sterile and 7 were male fertile. The remaining hybrids contained mitochondrial DNA that was identical to that of the ATR parent and all were male fertile.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 001962588

Download citation: RISBibTeXText

PMID: 24227255

DOI: 10.1007/bf00265310


Related references

Synthesis of male sterile triazine resistant brassica napus by somatic hybridization between cytoplasmic male sterile brassica oleracea and atrazine resistant brassica campestris. Theoretical & Applied Genetics 78(3): 445-455, 1989

Somatic hybridization of cytoplasmic male sterile Brassica oleracea and atrazine resistant B. campestris. Dissertation Abstracts International, B Sciences and Engineering 47(2): 455B, 1986

Atrazine-resistant cytoplasmic male-sterile-nigra broccoli obtained by protoplast fusion between cytoplasmic male-sterile Brassica oleracea and atrazine-resistant Brassica campestris. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 83(2): 201-208, 1991

Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male sterile brassica oleracea and atrazine resistant brassica campestris. Theoretical & Applied Genetics 74(3): 303-309, 1987

Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male-sterile Brassica oleracea and atrazine-resistant B. campestris. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 74(3): 303-309, 1987

Field testing of atrazine-resistant cytoplasmic male-sterile-nigra broccoli (Brassica oleracea L. var. italica) obtained by protoplast fusion. Journal of Genetics and Breeding 48(1): 33-40, 1994

Production of atrazine-resistant Brassica oleracea from somatic hybridization between B. oleracea and atrazine-resistant B. napus. Cruciferae Newsletter (12): 76-77, 1987

Atrazine resistant cauliflower obtained by somatic hybridization between brassica oleracea and atr brassica napus. Theoretical & Applied Genetics 78(2): 271-279, 1989

Atrazine-resistant cauliflower obtained by somatic hybridization between Brassica oleracea and ATR-B. napus. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 78(2): 271-279, 1989

Mitochondrial dna variation in cytoplasmic male sterile somatic hybrids of brassica napus. Plant Physiology & Biochemistry 25(3): 249-258, 1987

Production of the Ogura cytoplasmic male sterile (CMS) lines of Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis var. communis) and turnip (B. campestris L. ssp. rapifera) and cytological observation of their sterile organs. Acta Horticulturae Sinica 31(4): 467-471, 2004

Two new cytoplasmic male sterile lines of Brassica napus selected from interspecific hybridization. Journal of Huazhong Agricultural University 14(1): 21-25, 1995

Molecular identification of the sterile cytoplasm of NCa of a cytoplasmic male sterile line in rapeseed (Brassica napus L.). Scientia Agricultura Sinica 38(10): 1965-1972, 2005

Performance and genetic analysis of cytoplasmic male sterility of double-low cytoplasmic male sterile line 384A of Brassica napus L. Acta Agriculturae Boreali Sinica 13(4): 80-84, 1998

Comparison of brassica napus to brassica campestris and brassica oleracea based on the cytoplasmic characters cytoplasmic male sterility and chloroplast dna. Jarq 19(4): 253-258, 1985