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Synthesis of two storage proteins during larval development of the tobacco hornworm, Manduca sexta


, : Synthesis of two storage proteins during larval development of the tobacco hornworm, Manduca sexta. Developmental Biology, 1302: 671-681

Studies of synthesis and accumulation of the two storage proteins arylphorin and female-specific protein (FSP) during the final two larval instars of the tobacco hornworm showed both stage and temporal specificity. Arylphorin was present in both stages, but its synthesis ceased during the molt, during starvation, and at the wandering stage, and then resumed about 24 hr after the onset of feeding. During the larval molt about 25% of injected iodinated arylphorin was incorporated into the newly forming fifth instar cuticle. The cessation of arylphorin synthesis was mimicked by exposure of the fat body to 1 microgram/ml 20-hydroxyecdysone (20HE) in complete Grace's medium or to dilutions of Grace's medium greater than 50%. Lower concentrations of 20HE were ineffective, indicating that the cessation of synthesis in vivo was likely due to a combination of lack of excess nutrients and the hormonal milieu. The female-specific protein was not synthesized until the final larval instar, appearing first in females on Day 2 and later in males at the time of wandering, with synthesis continuing throughout the prepupal period. In vitro studies showed that this protein was synthesized as a 620-kDa protein, and then during secretion a 730-kDa immunoreactive form also appeared. Synthesis of FSP was inhibited by exposure of Day 2 fat body to 1 microgram/ml 20HE for 24 hr. Ligation followed by 20HE infusion showed that the disappearance of FSP from the hemolymph during the prepupal period was controlled by the rising ecdysteroid titer.

Accession: 001962649

PMID: 3197927

DOI: 10.1016/0012-1606(88)90359-4

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Related references

Webb, B.A.; Riddiford, L.M., 1988: Synthesis of two storage proteins during larval development of the tobacco hornworm, Manduca sexta. Studies of synthesis and accumulation of the two storage proteins arylphorin and female-specific protein (FSP) during the final two larval instars of the tobacco hornworm showed both stage and temporal specificity. Arylphorin was present in both s...

Soto, Pablo-Enrique-Jordan, 1970: Relationship of larval and adult food to the reproductive process of the tobacco hornworm, Manduca sexta (Johan.) and the tomato hornworm, Manduca quinquemaculata (Haw.)

Rosenthal, G.A.; Dahlman, D.L., 1988: Degradation of aberrant proteins by larval tobacco hornworm manduca sexta l. spingidae. The tobacco hornworm Manduca sexta (Sphingidae) readily incorporates L-canavanine, the L-2-amino-4-(guanidinooxy)butyric acid structural analog of L-arginine, into newly synthesized proteins. As a result, the developing fifth-instar larva produces...

Rosenthal, GA.; Dahlman, DL., 1988: Degradation of aberrant proteins by larval tobacco hornworm, Manduca sexta L. (Sphingidae). The tobacco hornworm Manduca sexta (Sphingidae) readily incorporates L-canavanine, the L-2-amino-4-(guanidinooxy)butyric acid structural analog of L-arginine, into newly synthesized proteins. As a result, the developing fifth-instar larva produces...

Wolfgang, W.J.; Riddiford, L.M., 1987: Cuticular mechanics during larval development of the tobacco hornworm, Manduca sexta. Tensile properties of the larval cuticle of the sphingid Manduca sexta were measured during the 5th instar. It was found that as the larvae grew and the cuticle thickened, the tangent modulus (intrinsic stiffness) for the cuticle declined rapidly....

Kaplanis, J.; Thompson, M.; Dutky, S.; Robbins, W.; Lindquist, E., 1972: The metabolism of 4-14 C-22, 25-bisdeoxyecdysone during larval development in the tobacco hornworm, Manduca sexta (L.). Hydroxylation and conjugation were the principal routes of metabolism for 4-14C-22,25-bisdeoxyecdysone when fed to tobacco hornworms during larval development. The major metabolite of 4-14C-22,25-bisdeoxyecdysone isolated from frass and also detec...

Kaplanis, J.N.; Thompson, M.J.; Dutky, S.R.; Robbins, W.E.; Lindquist, E.L., 1972: The metabolism of 4- 14 C-22, 25-bisdeoxyecdysone during larval development in the tobacco hornworm, Manduca sexta (L.). Steroids 20(1): 105-120

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Buckner, Js, 1982: Hormonal control of uric acid storage in the fat body during last-larval instar of Manduca sexta Tobacco hornworm. Journal of insect physiology8(12): 987-993

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