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Synthesis, cloning and sequencing of cDNA complementary to mRNA of prolactin from the human pituitary gland

, : Synthesis, cloning and sequencing of cDNA complementary to mRNA of prolactin from the human pituitary gland. Bioorganicheskaya Khimiya 13(12): 1687-1690

The poly(A)-containing mRNA from human pituitary and prolactinoma were purified and translated in a cell-free system prepared from rabbit reticulocytes. mRNA from prolactinoma was shown to be enriched with specific prolactin mRNA. DNA complementary to the prolactin mRNA from human pituitary was obtained and cloned. Sequencing of the 900-base pair insert by the Maxam-Gilbert technique suggested the cDNA coded for the previously published amino acid sequence.

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