EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Synthetic oligonucleotide hybridization probes to diagnose hop stunt viroid strains and citrus exocortis viroid


Journal of Virological Methods 19(2): 109-120
Synthetic oligonucleotide hybridization probes to diagnose hop stunt viroid strains and citrus exocortis viroid
Four species of synthetic oligonucleotide probes for the diagnosis of hop stunt viroid (HSV) and citrus viroid (CEV) were devised. Probe HSV-1 detected all the members of HSV group, such as HSV-hop, HSV-grapevine, HSV-cucumber, HSV-citrus and a viroid-like RNA isolated from plum trees affected by plum dapple fruit disease. Probe HSV-2 discriminated HSV-grapevine from the other members of HSV group. HSV-hop and HSV-grapevine consist of the same numbers of nucleotides, with only one nucleotide exchange. It was also shown that the two viroids were indistinguishable by their biological and physicochemical properties. However, by using probe HSV-2, HSV-hop and HSV-grapevine were apparently differentiated. Probe CEV-1 detected all the members of potato spindle tuber viroid (PSTV) group, such as PSTV, CEV and chrysanthemum stunt viroid (CSV). Probe CEV-2 discriminated CEV from the other members of PSTV group. It is thus emphasized that synthetic oligonucleotide probes are useful for the diagnosis of viroids and their related strains. It was discussed that the method can be used for the diagnosis of viruses and their related strains.

Accession: 001962690

PMID: 3366851

DOI: 10.1016/0166-0934(88)90154-1

Download PDF Full Text: Synthetic oligonucleotide hybridization probes to diagnose hop stunt viroid strains and citrus exocortis viroid



Related references

Synthesis of citrus exocortis viroid and chrysanthemum stunt viroid phosphorus 32 labelled complementary dna probes and detection by the probes. Virologica Sinica 1(4): 93-98, 1986

Detection of Multiple Infections of Citrus exocortis viroid, Citrus viroid III, and Hop stunt viroid Variants in Hunan Province, China. Plant disease: an international journal of applied plant pathology 91(9): 1205, 2007

Diagnosis of citrus exocortis viroid by synthetic oligonucleotide probes. Phytoparasitica 19(3): 264, 1991

First report of pospiviroids infecting ornamentals in the Netherlands: Citrus exocortis viroid in Verbena sp., Potato spindle tuber viroid in Brugmansia suaveolens and Solanum jasminoides, and Tomato apical stunt viroid in Cestrum sp. Plant Pathology 57(2): 399-399, 2008

Cytopathology of viroid-infected plant tissue. I. Alterations of plasmalemma and cell wall in Gynura aurantiaca after infection with the viroid of the exocortis disease of citrus citrus exocortis viroid. Phytopathologische Zeitschrift = Journal of phytopathology8(1): 1-18, 1980

Distribution of citrus exocortis viroid and hop stunt viroid in citrus orchards of central Italy as revealed by one-tube one-step RT-PCR. Phytopathologia Mediterranea 44(3): 322-326, 2005

Multiplex detection, distribution, and genetic diversity of Hop stunt viroid and Citrus exocortis viroid infecting citrus in Taiwan. Virology Journal 12(): 11-11, 2015

A mutual titer-enhancing relationship and similar localization patterns between Citrus exocortis viroid and Hop stunt viroid co-infecting two citrus cultivars. Virology Journal 12(): 142-142, 2016

Citrus exocortis viroid and Hop Stunt viroid doubly infecting grapevines in Brazil. Fitopatologia Brasileira 31(5): 440-446, 2006

First report of Hop stunt viroid and Citrus exocortis viroid on fig with symptoms of fig mosaic disease. Journal of Phytopathology (Berlin) 155(2): 125-128, 2007