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Synthetic oligonucleotide hybridization probes to diagnose hop stunt viroid strains and citrus exocortis viroid


, : Synthetic oligonucleotide hybridization probes to diagnose hop stunt viroid strains and citrus exocortis viroid. Journal of Virological Methods 19(2): 109-120

Four species of synthetic oligonucleotide probes for the diagnosis of hop stunt viroid (HSV) and citrus viroid (CEV) were devised. Probe HSV-1 detected all the members of HSV group, such as HSV-hop, HSV-grapevine, HSV-cucumber, HSV-citrus and a viroid-like RNA isolated from plum trees affected by plum dapple fruit disease. Probe HSV-2 discriminated HSV-grapevine from the other members of HSV group. HSV-hop and HSV-grapevine consist of the same numbers of nucleotides, with only one nucleotide exchange. It was also shown that the two viroids were indistinguishable by their biological and physicochemical properties. However, by using probe HSV-2, HSV-hop and HSV-grapevine were apparently differentiated. Probe CEV-1 detected all the members of potato spindle tuber viroid (PSTV) group, such as PSTV, CEV and chrysanthemum stunt viroid (CSV). Probe CEV-2 discriminated CEV from the other members of PSTV group. It is thus emphasized that synthetic oligonucleotide probes are useful for the diagnosis of viroids and their related strains. It was discussed that the method can be used for the diagnosis of viruses and their related strains.

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Accession: 001962690

PMID: 3366851

DOI: 10.1016/0166-0934(88)90154-1

PDF Full Text: Synthetic oligonucleotide hybridization probes to diagnose hop stunt viroid strains and citrus exocortis viroid


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Other references

HARDWICK, J.L., 1961: Isotope studies on the penetration of glucose into normal and carious enamel and dentine. Archives of Oral Biology 4: 97-100

Kolmos, J.H.; Nørskov, O.; Sørensen, J.S.; Undén, M., 1979: Tolerance of human low-macromolecular albumin infused rapidly to hemodialysis patients. Ugeskrift for Laeger 142(1): 18-20

Kalugina L. V., 1968: Cross section of Mikulino (Riss-Wuerm) interglacial sediments at the village of Fedosovo, Ivanovo region. A detailed stratigraphic section is given of Riss-Wuerm lacustrine and swamp sediments found in a borehole north of the village of Fedosovo (central European USSR).

Tague B.W.; Gerbi S.A., 1984: Processing of the large ribosomal rna precursor 2 proposed categories of rna rna interactions in eukaryotes. Journal of Molecular Evolution 20(3-4): 362-367

Hajek J.; Vrubel F.; Hanselova M.; Samohyl J., 1979: The use of sulfite gel for dilatation of male human urethra. Scripta Medica (Brno) 52(3): 147-152

Pack, A.I.; Galante, R.J.; Maislin, G.; Cater, J.; Metaxas, D.; Lu, S.; Zhang, L.; Von Smith, R.; Kay, T.; Lian, J.; Svenson, K.; Peters, L.L., 2006: Novel method for high-throughput phenotyping of sleep in mice. Assessment of sleep in mice currently requires initial implantation of chronic electrodes for assessment of electroencephalogram (EEG) and electromyogram (EMG) followed by time to recover from surgery. Hence, it is not ideal for high-throughput sc...

Chi, L.; Xu, Q.; Liang, X.; Wang, J.; Su, X., 2016: Iron-Based Metal-Organic Frameworks as Catalysts for Visible Light-Driven Water Oxidation. The development of earth-abundant, active, and stable catalysts is important for solar energy conversion. Metal-organic frameworks (MOFs) have been viewed as a promising class of porous materials, which may have innovative application in photocata...

Nagai, A., 1992: Study on ethane dimethane sulphonate (EDS)-induced spermatogenic damage and protective drugs against this damage in the rat. The effects of a single administration of ethane dimethane sulphonate (EDS), which has a direct cytotoxic effect on Leydig cells, was assessed for its spermatogenic damage and intratubular androgen level in SD male adult rats. The protective effec...

Altenburger, H.; Kroll, F.W., 1931: Chronaximetrische Ermudungsstudien. Chronaxie determinations were made on the hand extensors in fatigue. Irregular results were obtained in voluntary contraction. After strong faradic stimulation there was first a decrease of chronaxie from 056 [delta] to 0.1 [delta] after 3 min. st...

Konecki, J.; Jonek, J.J.; Gruszeczka, B., 1976: Some histochemical reactions in kidneys in experimental chest injuries. Polski Przeglad Chirurgiczny 48(6): 663-668