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T cell numbers and mitogenic responsiveness of peripheral blood mononuclear cells are decreased in copper deficient rats


Nutrition Research 10(7): 749-760
T cell numbers and mitogenic responsiveness of peripheral blood mononuclear cells are decreased in copper deficient rats
The effects of dietary copper (Cu) deficiency on the phenotypic profile and T cell mitogenic reactivity of mononuclear cells in rat blood were examined. Male Lewis rats were nursed by dams fed a diet containing adequate (6 .mu.g/g) or deficient (0.6 .mu.g/g) Cu and fed one of these diets for five weeks after weaning. Mononuclear cells were isolated from blood and analyzed by flow cytometry after immunolabelling with monoclonal antibodies to surface differentiation antigens. Total and differential leukocyte counts were not affected by dietary Cu. Decreased relative percentages and absolute numbers of T lymphocytes, CD4+ and CD8+ cells were present in the blood of rats deprived of adequate Cu during the lactation and postweaning periods. In contrast, the relative percentages of sIg+ (B) cells and monocytes, as well as the total number of monocytes, were higher in blood of the severely Cu deficient rats than in controls. The mononuclear blood cell profile for the postweaning model of Cu deficiency was similarly altered, although the percentages of CD8+ cells and monocytes were not significantly different from controls. Expression of class I (0X18) and class II (0X4) major histocompatibility antigens was not altered by Cu deficiency. In vitro reactivity of mononuclear cells from Cu deficient rats to nonspecific T cells mitogens was suppressed. The possibility that this decreased mitogenic responsiveness is due to both the lower number of responding T cells per well and abnormal interactions between antigen presenting cells and lymphocytes is discussed.

Accession: 001962946

DOI: 10.1016/S0271-5317(05)80824-3

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