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Use of nu/nu mice to model keratitis caused by free-living amoebae of the genus Acanthamoeba



Use of nu/nu mice to model keratitis caused by free-living amoebae of the genus Acanthamoeba



Comptes Rendus de l' Academie des Sciences Series 3, Sciences de la Vie 309(11): 499-503



The corneas of 8-week-old female NMRI nu/nu mice were injected with a suspension of 200 A. hatchetti cysts and trophozoites (at a ratio of 3:1) in 199 medium. 12 of 16 mice developed ocular pathology with keratitis on days 7-15. Disruption of the corneal fibres, neovascularization, and infiltration with polymorphonuclear cells were observed. Haematoxylin-eosin staining revealed trophozoites between the corneal fibres and very few cysts.

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Accession: 001997077

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