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Use of peduncle explants for high efficiency regeneration of Brassica oleracea L. - potential for transformation

Use of peduncle explants for high efficiency regeneration of Brassica oleracea L. - potential for transformation

Cruciferae Newsletter (13): 94-95

A procedure for regenerating peduncle explants is described. Of 97 explants from 10 cabbage peduncles, 82% regenerated after 2 weeks, of which 68% regenerated from the basal end, 4% from the apical end and 28% from both ends. The regeneration polarity was similar for broccoli Green Comet (85% regeneration), but with Chinese broccoli (88% regeneration), 53% of the explants produced buds at both ends, although buds were larger and more abundant at the basel end.

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Accession: 001997119

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