+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Use of the IDEIA ELISA to detect Chlamydia psittaci (ovis) in material from aborted fetal membranes and milk from ewes affected by ovine enzootic abortion

Use of the IDEIA ELISA to detect Chlamydia psittaci (ovis) in material from aborted fetal membranes and milk from ewes affected by ovine enzootic abortion

British Veterinary Journal 146(4): 364-367

The IDEIA ELISA was used to detect Chlamydia psittaci (ovis) antigen in ewes' milk to which were added serial dilutions of chlamydiae titrated as inclusion forming units (ifus) in McCoy cell tissue culture. The test was able to detect as few as 35 ifus/ml of the organism. The ELISA was then used to detect chlamydial antigen in fetal membranes and milk from ewes clinically affected with ovine enzootic abortion (OEA). The results were compared with results of isolation of chlamydiae in McCoy cell tissue culture from the same material. The fetal membranes of 17 of 19 ewes were positive for chlamydia when tested with the ELISA but chlamydia could be cultured from only 15 of them. Milk samples from 26 ewes which had aborted between 1 and 34 days previously were tested: chlamydiae could not be cultured from any of them and only one was positive when tested by the ELISA. The results show that the IDEIA ELISA is a sensitive test for the detection of C. psittaci (ovis) antigens. The positive results to this test for the three samples from which chlamydiae could not be cultured suggest that the test is not as specific as culture or that it detected dead organisms. Chlamydiae do not appear to be excreted in the milk of ewes affected with OEA.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 001997442

Download citation: RISBibTeXText

PMID: 2397377

DOI: 10.1016/s0007-1935(11)80031-x

Related references

Ovine enzootic abortion: field observations on naturally acquired and vaccine-elicited delayed type hypersensitivity to Chlamydia psittaci (ovis). Veterinary Record 118(12): 331-332, 1986

Diagnosis of ovine enzootic abortion, using a competitive ELISA based on monoclonal antibodies against variable segments 1 and 2 of the major outer membrane protein of Chlamydia psittaci serotype 1. American Journal of Veterinary Research 58(3): 228-235, 1997

Rapid detection of chlamydia psittaci in vaginal swabs of aborted ewes and goats by elisa. Veterinary Microbiology 11(3): 251-260, 1986

Chlamydia psittaci: is tonsillar tissue the portal of entry in ovine enzootic abortion?. Research in Veterinary Science 44(2): 260-261, 1988

Vaccination with temperature-sensitive mutant of Chlamydia psittaci against enzootic abortion of ewes. Veterinary Record 114(8): 193-194, 1984

Bacteriological diagnosis of ovine enzootic abortion by isolating Chlamydia psittaci in cell culture. Tierarztliche Umschau 42(2): 124...133, 1987

Direct isolation of the agent of enzootic abortion of ewes (Chlamydia psittaci) in cell cultures. Veterinary Record 113(18): 413-414, 1983

Isolation of Chlamydia psittaci from the genital tract of lambs: a possible link with enzootic abortion of ewes. Veterinary Record 123(15): 399-400, 1988

Efficacy against ovine enzootic abortion of an experimental vaccine containing purified elementary bodies of Chlamydia psittaci. Veterinary Microbiology 24(1): 21-27, 1990

Rapid detection of Chlamydia psittaci in vaginal swabs of aborted ewes and goats by enzyme linked immunosorbent assay (ELISA). Veterinary Microbiology 11(3): 251-259, 1986

A simple staining method for the identification of chlamydial elementary bodies in the fetal membranes of sheep affected by ovine enzootic abortion. Veterinary Microbiology 21(3): 233-240, 1990

Detection of antigens of the ovine enzootic abortion agent (Chlamydia ovis) by the immunoperoxidase method. Veterinariya, Moscow (10): 38-39, 1975

Development and evaluation of an indirect ELISA to detect antibodies to abortion strains of Chlamydia psittaci in sheep sera. Veterinary Microbiology 43(1): 1-12, 1995

Evaluation of the polymerase chain reaction (PCR) for detection of Chlamydia psittaci in abortion material from ewes. Zentralblatt fur Bakteriologie 277(4): 446-453, 1992

Common abortion diseases of sheep Bacterial diseases include vibriosis from Campylobacter fetus var. fetus, enzootic abortion of ewes (EAE) from Chlamydia ovis, salmonellosis from Salmonella. California veterinarian 36(9): 21-23, 1982