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Use of the laser microbeam for preserving frozen Drosophila embryos

Use of the laser microbeam for preserving frozen Drosophila embryos

Zoological Science (Tokyo) 6.3: 499-505

The possibility of using the laser microbeam for preserving early embryos of Drosophila melanogaster at -196 degrees C was examined. To date, the most difficult problem has involved methods for introducing protective agents into the eggs through the vitelline membrane; in the present study a UV laser beam was applied to inflict breaks or cuts on the micropyle to allow protective glycerol to enter.

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Accession: 001997510

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