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Use of the nisin resistance determinant as a selectable marker for a food-grade cloning vector



Use of the nisin resistance determinant as a selectable marker for a food-grade cloning vector



Journal of Dairy Science 72(Suppl. 1): 115



The 7.6-kilobase (kb) plasmid, pFM011, encoding the nisin resistance (Nisr) phenotype, was evaluated for its ability to serve as a vector for cloning homologous DNA into lactococci. A selective medium, designated M17-GTN, consisting of M17-glucose agar (pH 7) containing 0.1% Tween 20 and 20-40 IU nisin/ml, was employed to isolate Nisr Lactococcus lactis var. lactis LM0230 cells containing pFM011.

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Accession: 001997528

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