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Use of two-colour flow cytometry to assess killing of Giardia muris trophozoites by antibody and complement

Use of two-colour flow cytometry to assess killing of Giardia muris trophozoites by antibody and complement

Parasitology 99 Pt 2: 199-203

A two-colour flow cytometry technique was used to assess killing of Giardia muris trophozoites by rabbit anti-trophozoite antibodies and complement. Binding of rabbit antibody to trophozoites and killing of torphozoites were documented by flow cytometry, after incubating the organisms with fluorescein-conjugated anti-rabbit IgG and propidium iodide (PI). Percentages of PI+ (dead) trophozoites ranged from > 80%, after incubation with rabbit antiserum and complement, to < 30%, after incubation with complement alone. The assay technique may be applicable to studies aimed at determining whether intestinal antibodies from Giardia-infected mammals can kill Giardia trophozoites.

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Accession: 001997612

Download citation: RISBibTeXText

PMID: 2594412

DOI: 10.1017/s0031182000058649

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