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Usefulness of fibroblast culture for testing of cattle tissues polluted with heavy metals

Usefulness of fibroblast culture for testing of cattle tissues polluted with heavy metals

Environmental Research 51(2): 163-169

Cattle tissues (liver, kidney, brain, and lung) that had been polluted with heavy metals were tested for their ability to alter fibroblast culture growth, cellular protein and DNA content, and fibroblast DNA synthesis. At 72 hr of incubation a significant increase in cellular DNA and [14C]thymidine incorporation was noted in the primary cultures as well as in the subcultures compared to controls. Fibroblast cultures also displayed growth inhibition and reduction in protein content. The measurement of basic biochemical parameters of the fibroblast culture may represent a sensitive means of assessing rapidly the activity of heavy metals deposited in the tissues of cattle as a result of their grazing on polluted soil.

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Accession: 001997714

Download citation: RISBibTeXText

PMID: 2335157

DOI: 10.1016/s0013-9351(05)80086-6

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