EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

A short incubation serum neutralization test for bovine viral diarrhea virus


A short incubation serum neutralization test for bovine viral diarrhea virus



Canadian Journal of Veterinary Research 56(2): 161-164



ISSN/ISBN: 0830-9000

PMID: 1317246

A three day serum neutralization (SN) test for the detection of antibodies to bovine viral diarrhea virus (BVDV), which is an improvement on the existing five day test, is described. The improved test results in a more rapid viral cytopathic effect and utilizes Madin Darby kidney (MDBK) cells, and horse serum as a medium supplement. A comparison of tests utilizing the NADL and the Singer strains of BVDV and the use of either secondary bovine kidney cells with calf serum (BKCS) or continuous MDBK cells with horse serum (MDHS) was performed. Analysis of the SN results of 685 serum samples from 445 Quebec and Ontario cattle showed that there was no difference, as expected, in the means of the SN antibody titers when the NADL strain was used in either the BKCS or MDHS system but SN antibody titers were elevated (p less than 0.01) when the Singer strain was used in the MDHS system. The SN test with the Singer strain also yielded significantly higher titers for sera from 200 Alberta cattle.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002014833

Download citation: RISBibTeXText

Related references

Effects of incubation temperatures and bovine viral diarrhea virus immune serum on bovine turbinate cells persistently infected with a noncytopathogenic isolate of bovine viral diarrhea virus. American Journal of Veterinary Research 42(4): 647-649, 1981

Detection of Bovine viral diarrhea virus-specific neutralizing antibodies in fresh colostrum: a modification of the virus neutralization test. Journal of Veterinary Diagnostic Investigation: Official Publication of the American Association of Veterinary Laboratory Diagnosticians Inc 25(2): 263-266, 2013

Neutralization kinetics of bovine viral diarrhea virus by hyperimmune serum: one or multi-hit mechanism. Comparative Immunology Microbiology and Infectious Diseases 9(1): 37-45, 1986

Effect of virus/serum incubation period (1 and 24 hours) on the sensitivity and specificity of the serum neutralization test for infectious bovine rhinotracheitis. Revista Cientifica, Facultad de Ciencias Veterinarias, Universidad del Zulia 14(3): 213-216, 2004

Single dilution Avidity-Blocking ELISA as an alternative to the Bovine Viral Diarrhea Virus neutralization test. Journal of Virological Methods 175(2): 228-235, 2011

Evaluation of a single dilution ELISA system for detection of seroconversion to bovine viral diarrhea virus, bovine respiratory syncytial virus, parainfluenza-3 virus, and infectious bovine rhinotracheitis virus: comparison with testing by virus neutralization and hemagglutination inhibition. Journal of Veterinary Diagnostic Investigation: Official Publication of the American Association of Veterinary Laboratory Diagnosticians Inc 10(1): 43-48, 1998

Comparison of Neutralization Peroxidase-Linked Antibody (NPLA) assay and Serum Neutralization (SN) test for detection of antibodies to bovine viral diarrhoea (BVD) virus in cattle sera. Veteriner Fakultesi Dergisi, Ankara Universitesi 46(2/3): 249-255, 1999

Response of cattle persistently infected with noncytopathic bovine viral diarrhea virus to vaccination for bovine viral diarrhea and to subsequent challenge exposure with cytopathic bovine viral diarrhea virus. American Journal of Veterinary Research 46(12): 2467-2470, 1985

Methods for detection and frequency of contamination of fetal calf serum with bovine viral diarrhea virus and antibodies against bovine viral diarrhea virus. Journal of Veterinary Diagnostic Investigation: Official Publication of the American Association of Veterinary Laboratory Diagnosticians Inc 3(3): 199-203, 1991

Measuring bovine viral diarrhea virus vaccine response: using a commercially available ELISA as a surrogate for serum neutralization assays. Vaccine 30(46): 6559-6563, 2012

Effect of vaccination of pregnant beef heifers on the concentrations of serum IgG and specific antibodies to bovine herpesvirus 1, bovine viral diarrhea virus 1, and bovine viral diarrhea virus 2 in heifers and calves. Canadian Journal of Veterinary Research 83(4): 313-316, 2019

An immunodiffusion test for detection of bovine viral diarrhea virus antibodies in bovine serum. American Journal of Veterinary Research 40(8): 1189-1191, 1979

Comparison of levels and duration of detection of antibodies to bovine viral diarrhea virus 1, bovine viral diarrhea virus 2, bovine respiratory syncytial virus, bovine herpesvirus 1, and bovine parainfluenza virus 3 in calves fed maternal colostrum or a colostrum-replacement product. Canadian Journal of Veterinary Research 78(2): 81-88, 2014

The microculture neutralization test and the short period security test of prevention Bovine viral Diarrhoea: Mucosal disease with attenuated vaccine of hog cholera virus. Sichuan Daxue Xuebao (Ziran Kexueban) 40(5): 970-973, 2003

Viral and viral protein specificity of antibodies induced in cows persistently infected with noncytopathic bovine viral diarrhea virus after vaccination with cytopathic bovine viral diarrhea virus. American Journal of Veterinary Research 49(7): 1040-1044, 1988